ArticleCroatian medical journal2014
Reliability of long vs short COI markers in identification of forensically important flies.
Article in Croatian medical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Virome analysis and detection of ticks and tick-borne viruses in Shanghai, China.Frontiers in microbiology · 2025Article
- Longer mtDNA Fragments Provide a Better Insight into the Genetic Diversity of the Sycamore Lace Bug,Insects · 2022Article
- Article
- Improved phylogeny of brown algae Cystoseira (Fucales) from the Atlantic-Mediterranean region based on mitochondrial sequences.PloS one · 2019Article
- Article
- Article
- A brief review of forensically important flesh flies (Diptera: Sarcophagidae).Forensic sciences research · 2018Review
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1 author.
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Abstract
aimTo compare the reliability of short and long cytochrome oxidase I gene fragment (COI) in identification of forensically important Diptera from Egypt and China.
methodsWe analyzed 50 specimens belonging to 18 species. The two investigated markers were amplified by polymerase chain reaction (PCR) followed by direct sequencing. Nucleotide sequence divergences were calculated using the Kimura two-parameter (K2P) distance model and neighbor-joining (NJ) phylogenetic trees.
resultsAlthough both tested fragments showed an overlap between intra and interspecific variations, long marker had greater completeness of monophyletic separation with high bootstrap support. Moreover, NJ tree based on the long fragment clustered species more in accordance with their taxonomic classification than that based on the short fragment.
conclusionIn dipterous identification, it is recommended to use the long COI marker due to its greater reliability and safety.
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