ReviewNature methods2014
Fluorophore localization algorithms for super-resolution microscopy.
Review in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 115 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
115 citing papers in PubMed.
- The multifaceted significance of phosphoinositides in endocytic trafficking.FEBS letters · 2026Review
- ONE microscopy.Nature protocols · 2026Review
- BlinkFusion: modular platform quantifying labeling efficiency and photophysics in regular and super-resolution fluorescence microscopy.Frontiers in bioinformatics · 2026Article
- Optical Approaches to Dissect the Structure and Dynamics of the Synapse at Nanoscale Resolution.Advances in neurobiology · 2026Review
- Multiparametric super-resolution optoacoustic imaging with ICG-tagged microbubbles.Materials today. Bio · 2025Article
- Cancellation of Spectral and Spatial Crosstalk in Spectral Imaging for High-Dynamic-Range Electrophoretic Analysis of STR-PCR Products.Analytical chemistry · 2025Article
- Semi-automated Analysis of Beading in Degenerating Axons.Neuroinformatics · 2025Article
- GJFocuser: a Gaussian difference and joint learning-based autofocus method for whole slide imaging.Biomedical optics express · 2025Article
- Measurement precision bounds on aberrated single-molecule emission patterns.Optics express · 2024Article
- 3D Traction Force Microscopy in Biological Gels: From Single Cells to Multicellular Spheroids.Annual review of biomedical engineering · 2024Review
- Evaluating the resolution of conventional optical microscopes through point spread function measurement.iScience · 2023Review
- Trends in Single-Molecule Total Internal Reflection Fluorescence Imaging and Their Biological Applications with Lab-on-a-Chip Technology.Sensors (Basel, Switzerland) · 2023Review
- Single-molecule tracking to determine the abundances and stoichiometries of freely-diffusing protein complexes in living cells: Past applications and future prospects.The Journal of chemical physics · 2023Review
- Recognition of Single Fluorescence Events by Temporal Pixel Intensity Fluctuation.Chemical & biomedical imaging · 2023Article
- High-Throughput Single-Molecule Sensors: How Can the Signals Be Analyzed in Real Time for Achieving Real-Time Continuous Biosensing?ACS sensors · 2023Article
- Super-resolution with Binary Priors: Theory and Algorithms.IEEE transactions on signal processing : a publication of the IEEE Signal Processing Society · 2023Article
- Three-dimensional imaging of biological cells using surface plasmon coupled emission.Journal of biomedical optics · 2022Article
- Super-Resolution Displacement Spectroscopic Sensing over a Surface "Rainbow".Engineering (Beijing, China) · 2022Article
- Optical quantum super-resolution imaging and hypothesis testing.Nature communications · 2022Article
- Prior information improves resolution.Biophysical journal · 2022Article
55 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Super-resolution localization microscopy methods provide powerful new capabilities for probing biology at the nanometer scale via fluorescence. These methods rely on two key innovations: switchable fluorophores (which blink on and off and can be sequentially imaged) and powerful localization algorithms (which estimate the positions of the fluorophores in the images). These techniques have spurred a flurry of innovation in algorithm development over the last several years. In this Review, we survey the fundamental issues for single-fluorophore fitting routines, localization algorithms based on principles other than fitting, three-dimensional imaging, dipole imaging and techniques for estimating fluorophore positions from images of multiple activated fluorophores. We offer practical advice for users and adopters of algorithms, and we identify areas for further development.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.