Evidence map›Paper›PMID 24577276›Full record

ReviewNature methods2014

Precisely and accurately localizing single emitters in fluorescence microscopy.

Hendrik Deschout, Francesca Cella Zanacchi, Michael Mlodzianoski, Alberto Diaspro, Joerg Bewersdorf, Samuel T Hess, Kevin Braeckmans

Abstract readReview
PubMed Publisher
In one paragraph

Review in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.

0numbers the graph read from it
0cells of the map it votes in
181citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

181 citing papers in PubMed.

  1. Article
  2. Review
  3. From Microscale to Nanoscale Shadow Electrochemiluminescence Microscopy.Angewandte Chemie (International ed. in English) · 2026
    Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. A Versatile Drift-Free Super-Resolution Imaging Method via Oblique Bright-Field Correlation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Spectral precision improvement with demagnifying spectral images in spectroscopic nanoscopy.Journal of the Optical Society of America. A, Optics, image science, and vision · 2024
    Article
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article

121 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Hendrik Deschout1] Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium. [2] Center for Nano- and Biophotonics, Ghent University, Ghent, Belgium.
Francesca Cella Zanacchi1] Department of Nanophysics, Italian Institute of Technology, Genoa, Italy. [2] Department of Physics, University of Genoa, Genoa, Italy.
Michael MlodzianoskiDepartment of Physics and Astronomy, University of Maine, Orono, Maine, USA.
Alberto Diaspro1] Department of Nanophysics, Italian Institute of Technology, Genoa, Italy. [2] Department of Physics, University of Genoa, Genoa, Italy.ORCID 0000000249165928
Joerg Bewersdorf1] Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA. [2] Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA.
Samuel T HessDepartment of Physics and Astronomy, University of Maine, Orono, Maine, USA.
Kevin Braeckmans1] Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium. [2] Center for Nano- and Biophotonics, Ghent University, Ghent, Belgium.

Funding

The Role of Rafts in Virus-Induced Membrane RemodelingK25AI065459 · NIAID · UNIVERSITY OF MAINE ORONO · PI HESS, SAMUEL T · 2005 to 2009
$663k
The Role of the Actin Cytoskeleton and Cholesterol in Influenza Virus AssemblyR15GM094713 · NIGMS · UNIVERSITY OF MAINE ORONO · PI HESS, SAMUEL T · 2010 to 2010
$425k
NIAID NIH HHS K25 AI065459NIGMS NIH HHS R15 GM094713NIGMS NIH HHS R15-GM094713
6 · The paper itself

Abstract

Methods based on single-molecule localization and photophysics have brought nanoscale imaging with visible light into reach. This has enabled single-particle tracking applications for studying the dynamics of molecules and nanoparticles and contributed to the recent revolution in super-resolution localization microscopy techniques. Crucial to the optimization of such methods are the precision and accuracy with which single fluorophores and nanoparticles can be localized. We present a lucid synthesis of the developments on this localization precision and accuracy and their practical implications in order to guide the increasing number of researchers using single-particle tracking and super-resolution localization microscopy.

Indexed as

Fluorescent DyesMicroscopy, FluorescenceReproducibility of ResultsFluorescent Dyes

Identifiers

PMID24577276

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.