ArticleBiochemistry2014
Strength of axial water ligation in substrate-free cytochrome P450s is isoform dependent.
Article in Biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- A Molecular Mechanism for the Reduction of the Cu Site in Lytic Polysaccharide Monooxygenases by Phenol Reductants.JACS Au · 2025Article
- De Novo design of α-helical heme binding proteins capable of versatile cofactor ligation.Methods in enzymology · 2025Article
- Catalysis and Electron Transfer inChemical reviews · 2022Review
- The pH-Induced Selectivity Between Cysteine or Histidine Coordinated Heme in an Artificial α-Helical Metalloprotein.Angewandte Chemie (International ed. in English) · 2021Article
- Characterization of CYP125A13, the First Steroid C-27 Monooxygenase fromJournal of microbiology and biotechnology · 2020Article
- Multiple drug binding modes in Mycobacterium tuberculosis CYP51B1.Journal of inorganic biochemistry · 2020Article
- Clobetasol Propionate Is a Heme-Mediated Selective Inhibitor of Human Cytochrome P450 3A5.Journal of medicinal chemistry · 2020Article
- Substrate-Assisted Hydroxylation andACS catalysis · 2020Article
- Binding of a physiological substrate causes large-scale conformational reorganization in cytochrome P450 51.The Journal of biological chemistry · 2018Article
- CW EPR parameters reveal cytochrome P450 ligand binding modes.Journal of inorganic biochemistry · 2018Article
- Probing Ligand Exchange in the P450 Enzyme CYP121 from Mycobacterium tuberculosis: Dynamic Equilibrium of the Distal Heme Ligand as a Function of pH and Temperature.Journal of the American Chemical Society · 2017Article
- Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome bChemical communications (Cambridge, England) · 2017Article
- The tetrahydrobiopterin radical interacting with high- and low-spin heme in neuronal nitric oxide synthase - A new indicator of the extent of NOS coupling.Free radical biology & medicine · 2016Article
- Drug modulation of water-heme interactions in low-spin P450 complexes of CYP2C9d and CYP125A1.Biochemistry · 2015Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
The heme-containing cytochrome P450s exhibit isoform-dependent ferric spin equilibria in the resting state and differential substrate-dependent spin equilibria. The basis for these differences is not well understood. Here, magnetic circular dichroism (MCD) reveals significant differences in the resting low spin ligand field of CYPs 3A4, 2E1, 2C9, 125A1, and 51B1, which indicates differences in the strength of axial water ligation to the heme. The near-infrared bands that specifically correspond to charge-transfer porphyrin-to-metal transitions span a range of energies of nearly 2 kcal/mol. In addition, the experimentally determined MCD bands are not entirely in agreement with the expected MCD energies calculated from electron paramagnetic resonance parameters, thus emphasizing the need for the experimental data. MCD marker bands of the high spin heme between 500 and 680 nm were also measured and suggest only a narrow range of energies for this ensemble of high spin Cys(S(-)) → Fe(3+) transitions among these isoforms. The differences in axial ligand energies between CYP isoforms of the low spin states likely contribute to the energetics of substrate-dependent spin state perturbation. However, these ligand field energies do not correlate with the fraction of high spin vs low spin in the resting state enzyme, suggestive of differences in water access to the heme or isoform-dependent differences in the substrate-free high spin states as well.
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