Evidence map›Paper›PMID 24575334›Full record

ArticleBiomedical optics express2014

Dynamic autofluorescence imaging of intracellular components inside living cells using direct electron beam excitation.

Yasunori Nawa, Wataru Inami, Aki Miyake, Atsushi Ono, Yoshimasa Kawata, Sheng Lin, Susumu Terakawa

Open access · goldAbstract read
In one paragraph

Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
4.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yasunori NawaGraduate School of Science and Technology, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; Research Fellow of the Japan Society for the Promotion of Science, Chiyoda, Tokyo 102-0083, Japan.
Wataru InamiFaculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; CREST, Japan Science and Technology Agency, Japan.
Aki MiyakeFaculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; CREST, Japan Science and Technology Agency, Japan.
Atsushi OnoCREST, Japan Science and Technology Agency, Japan ; Research Institute of Electronics, Shizuoka University, Johoku, Naka, Hamamatsu 4328011, Japan.
Yoshimasa KawataGraduate School of Science and Technology, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; Faculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan ; CREST, Japan Science and Technology Agency, Japan ; Research Institute of Electronics, Shizuoka University, Johoku, Naka, Hamamatsu 4328011, Japan.
Sheng LinFaculty of Engineering, Shizuoka University, Johoku, Naka, Hamamatsu 4328561, Japan.
Susumu TerakawaCREST, Japan Science and Technology Agency, Japan ; Photon Medical Research Center, Hamamatsu University School of Medicine, Handayama, Higashi, Hamamatsu 4313192, Japan.
Shizuoka University · JPHamamatsu University School of Medicine · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a high-resolution fluorescence microscope in which fluorescent materials are directly excited using a focused electron beam. Electron beam excitation enables detailed observations on the nanometer scale. Real-time live-cell observation is also possible using a thin film to separate the environment under study from the vacuum region required for electron beam propagation. In this study, we demonstrated observation of cellular components by autofluorescence excited with a focused electron beam and performed dynamic observations of intracellular granules. Since autofluorescence is associated with endogenous substances in cells, this microscope can also be used to investigate the intrinsic properties of organelles.

Indexed as

(110.0180) Microscopy(170.0180) Microscopy(170.2520) Fluorescence microscopy(180.2520) Fluorescence microscopy(180.5810) Scanning microscopy

Identifiers

PMID24575334
PMCPMC3920870
OpenAlexW2077745269

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.