ArticleTropical life sciences research2010
The Stability of rRNA in Heat-killed Salmonella enterica Cells and Its Detection by Fluorescent In Situ Hybridisation (FISH).
Article in Tropical life sciences research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed, 9 citations in OpenAlex.
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Differential ratio amplicons (REnvironmental microbiology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Differentiation of viable cells from non-viable cells is a major concern in the detection of foodborne microbial pathogens. Fluorescent in situ hybridisation (FISH) has been utilised as a promising method in this regard. The ability of FISH to differentiate viable cells from non-viable cells depends on the rapid degradation of rRNA in non-viable cells. In our work, Salmonella enterica that were heat-killed at 80°C, 100°C and 121°C were examined for the presence of rRNA using FISH at various times ranging from 5 minutes to 48 hours after heat treatment. rRNA was detected by FISH in heat-killed bacteria for 12 hours, 3 hours and 1 hour after treatment at 80°C, 100°C and 121°C, respectively. These results indicate that there is a correlation between temperature and stability of rRNA in heat-killed bacteria. In conclusion, FISH was determined to be a suitable method for differentiation of viable cells from non-viable cells, especially for samples subjected to extreme heat.
Indexed as
Identifiers
24575189PMC3819069W2106181328What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.