ArticleJournal of oral and maxillofacial pathology : JOMFP2013
Ca(2+)-deposition in cell matrix correlates significantly with osteocalcin-expression in osteogenic differentiated ATSC: Even in a coculture system with HUVEC.
Article in Journal of oral and maxillofacial pathology : JOMFP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Metformin carbon dots-based osteogenic and protein delivery system to promote bone regeneration in periodontitis.Bioactive materials · 2025Article
- Effects of mangosteen peel extract combined with demineralized freeze-dried bovine bone xenograft on osteocalcin, collagen 1, and osteoblast as alveolar bone regeneration in socket preservation.Journal of Indian Prosthodontic SocietyArticle
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Abstract
backgroundTissue engineering offers the means for replacing or repairing diseased organs within the patient's body. The current problem in its clinical use is sufficient and fast revascularization of the transplanted tissues. The idea of bone-reconstruction deals with three-dimensional bone equivalents that are composed of endothelial cells (ECs) and adipose tissue derived stromal cells (ATSCs) showing osteogenic differentiation. MATERIALS AND
methodsATSC were isolated, cultivated until third passage and osteogenically differentiated by 1.25-dihydroxycholecalciferol. Coculture systems with human umbilical vein endothelial cells (HUVEC) were performed. Osteogenic differentiation was analyzed in FACS-analyses (n = 7), by the measurement of Ca(2+)-deposition in the cell matrix (marker for osteogenic differentiation) and the expression of alkaline phosphatase (AP).
resultsCa(2+)-deposition in the cell matrix and osteocalcin-expression correlated significantly (P = 0.030) during osteogenic differentiation (n = 7). The osteogenic cell differentiated ATSC in the coculture system (n = 6) even showed a clear increase of Ca(2+)-deposition. The time of starting the coculture did not influence the differentiation. Measurement of the Ca(2+)-deposition correlates significantly to the osteogenic differentiation and osteocalcin-expression.
conclusionATSC are a promising source for bone tissue engineering. They can be differentiated into osteoblasts in a coculture system with HUVEC without the loss of any differentiation capacity. For bone tissue-equivalent fabrication, this is an encouraging procedure that is feasible and provides fast revascularization of the bone-equivalents.
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