ArticleMolecular biology of the cell2014
FAP20 is an inner junction protein of doublet microtubules essential for both the planar asymmetrical waveform and stability of flagella in Chlamydomonas.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 95 citations in OpenAlex.
- Loss-of-function variants in SAXO6, encoding a microtubule inner protein of photoreceptor cilia, cause a late-onset retinal dystrophy.American journal of human genetics · 2026Article
- Doublet microtubule-associated tektins and enzymes differentially regulate sperm flagellar integrity and motility.Nature communications · 2026Article
- Microtubule inner proteins ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity.Nature communications · 2024Article
- FAP20 is required for flagellum assembly inMolecular biology of the cell · 2024Article
- The Structure of Cilium Inner Junctions Revealed by Electron Cryo-tomography.bioRxiv : the preprint server for biology · 2024Article
- Poc1 bridges basal body inner junctions to promote triplet microtubule integrity and connections.The Journal of cell biology · 2024Article
- Neurons dispose of hyperactive kinesin into glial cells for clearance.The EMBO journal · 2024Article
- The MBO2/FAP58 heterodimer stabilizes assembly of inner arm dyneinMolecular biology of the cell · 2024Article
- Article
- MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement.Developmental cell · 2024Article
- FAP20 is required for flagellum assembly inbioRxiv : the preprint server for biology · 2024Article
- Review
- Doublet microtubule inner junction protein FAP20 recruits tubulin to the microtubule lattice.Structure (London, England : 1993) · 2023Article
- Poc1 is a basal body inner junction protein that promotes triplet microtubule integrity and interconnections.bioRxiv : the preprint server for biology · 2023Article
- FAP106 is an interaction hub for assembling microtubule inner proteins at the cilium inner junction.Nature communications · 2023Article
- Modulation of inner junction proteins contributes to axoneme differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins.Nature communications · 2023Article
- The inner junction protein CFAP20 functions in motile and non-motile cilia and is critical for vision.Nature communications · 2022Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
The axoneme-the conserved core of eukaryotic cilia and flagella-contains highly specialized doublet microtubules (DMTs). A long-standing question is what protein(s) compose the junctions between two tubules in DMT. Here we identify a highly conserved flagellar-associated protein (FAP), FAP20, as an inner junction (IJ) component. The flagella of Chlamydomonas FAP20 mutants have normal length but beat with an abnormal symmetrical three-dimensional pattern. In addition, the mutant axonemes are liable to disintegrate during beating, implying that interdoublet connections may be weakened. Conventional electron microscopy shows that the mutant axonemes lack the IJ, and cryo-electron tomography combined with a structural labeling method reveals that the labeled FAP20 localizes at the IJ. The mutant axonemes also lack doublet-specific beak structures, which are localized in the proximal portion of the axoneme and may be involved in planar asymmetric flagellar bending. FAP20 itself, however, may not be a beak component, because uniform localization of FAP20 along the entire length of all nine DMTs is inconsistent with the beak's localization. FAP20 is the first confirmed component of the IJ. Our data also suggest that the IJ is important for both stabilizing the axoneme and scaffolding intra-B-tubular substructures required for a planar asymmetrical waveform.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.