Evidence map›Paper›PMID 24573178›Full record

ArticleInternational journal of molecular medicine2014

Biodistribution of mesenchymal stem cell-derived extracellular vesicles in a model of acute kidney injury monitored by optical imaging.

Cristina Grange, Marta Tapparo, Stefania Bruno, Devasis Chatterjee, Peter J Quesenberry, Ciro Tetta, Giovanni Camussi

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 221 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
221citing papers in PubMed, 2 pooled it
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

221 citing papers in PubMed, 2 syntheses or guidelines pooled it, 357 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
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  6. Review
  7. Article
  8. Article
  9. Integrative Approaches to Treating Cellular Senescence in Kidney Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  10. Article
  11. Review
  12. Review
  13. 80 years of extracellular vesicles: from discovery to clinical translation.Extracellular vesicles and circulating nucleic acids · 2026
    Review
  14. Article
  15. Review
  16. Review
  17. Article
  18. Review
  19. Article
  20. Article

161 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Cristina GrangeDepartment of Medical Sciences, University of Torino, Torino, Italy.
Marta TapparoTranslational Center for Regenerative Medicine, University of Torino, Torino, Italy.
Stefania BrunoDepartment of Molecular Biotechnology and Health Science, University of Torino, Torino, Italy.
Devasis ChatterjeeDepartment of Medicine, The Warren Alpert Medical School of Brown University, Providence, RI, USA.
Peter J QuesenberryDepartment of Medicine, The Warren Alpert Medical School of Brown University, Providence, RI, USA.
Ciro TettaEMEA LA Medical Board, Fresenius Medical Care, Bad Homburg, Germany.
Giovanni CamussiDepartment of Medical Sciences, University of Torino, Torino, Italy.
University of Turin · ITBrown University · USFresenius Medical Care (Germany) · DE

Funding

Regulation of renal and bone marrow injury by extracellular vesicle non-coding RNUH3TR000880 · NCATS · RHODE ISLAND HOSPITAL · PI QUESENBERRY, PETER J. · 2015 to 2017
$1.1M
Regulation of renal and bone marrow injury by extracellular vesicle non-coding RNUH2TR000880 · NCATS · RHODE ISLAND HOSPITAL · PI QUESENBERRY, PETER J. · 2013 to 2014
$498k
NCATS NIH HHS UH2 TR000880NCATS NIH HHS UH2TR000880NCATS NIH HHS UH3 TR000880
6 · The paper itself

Abstract

Mesenchymal stem cells (MSCs) contribute to the recovery of tissue injury, providing a paracrine support. Cell-derived extracellular vesicles (EVs), carrying membrane and cytoplasmatic constituents of the cell of origin, have been described as a fundamental mechanism of intercellular communication. We previously demonstrated that EVs derived from human MSCs accelerated recovery following acute kidney injury (AKI) in vivo. The aim of the present study was to investigate the biodistribution and the renal localization of EVs in AKI. For this purpose, two methods for EV labeling suitable for in vivo tracking with optical imaging (OI), were employed using near infrared (NIR) dye (DiD): i) labeled EVs were generated by MSCs pre-incubated with NIR dye and collected from cell supernatants; ii) purified EVs were directly labeled with NIR dye. EVs obtained with these two procedures were injected intravenously (i.v.) into mice with glycerol-induced AKI and into healthy mice to compare the efficacy of the two labeling methods for in vivo detection of EVs at the site of damage. We found that the labeled EVs accumulated specifically in the kidneys of the mice with AKI compared with the healthy controls. After 5 h, the EVs were detectable in whole body images and in dissected kidneys by OI with both types of labeling procedures. The directly labeled EVs showed a higher and brighter fluorescence compared with the labeled EVs produced by cells. The signal generated by the directly labeled EVs was maintained in time, but provided a higher background than that of the labeled EVs produced by cells. The comparison of the two methods indicated that the latter displayed a greater specificity for the injured kidney.

Indexed as

Acute Kidney InjuryAnimalsHumansMesenchymal Stem CellsMiceMice, NudeSecretory Vesicles

Identifiers

PMID24573178
PMCPMC4020482
OpenAlexW2146668660

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.