Evidence map›Paper›PMID 24572459›Full record

ReviewJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2014

Analysis of biomolecular interactions using affinity microcolumns: a review.

Xiwei Zheng, Zhao Li, Sandya Beeram, Maria Podariu, Ryan Matsuda, Erika L Pfaunmiller, Christopher J White, NaTasha Carter, David S Hage

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed, 77 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Characterization of binding by sulfonylureas with normal or modified human serum albumin using affinity microcolumns prepared by entrapment.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2023
    Article
  6. Article
  7. Review
  8. Review
  9. Glycoprotein analysis using lectin microcolumns and capillary electrophoresis: Characterization of alphaJournal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2021
    Article
  10. Characterization of drug binding with alphaJournal of chromatography. A · 2021
    Article
  11. Article
  12. Article
  13. Affinity chromatography: A review of trends and developments over the past 50 years.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2020
    Review
  14. Buffer Influence on the Amino Acid Silica Interaction.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020
    Article
  15. Article
  16. Optimization of protein entrapment in affinity microcolumns using hydrazide-activated silica and glycogen as a capping agent.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2019
    Article
  17. Review
  18. Kinetic Analysis by Affinity Chromatography.Frontiers in chemistry · 2019
    Review
  19. Chromatographic studies of chlorpropamide interactions with normal and glycated human serum albumin based on affinity microcolumns.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2018
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Xiwei ZhengDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Zhao LiDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Sandya BeeramDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Maria PodariuDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Ryan MatsudaDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Erika L PfaunmillerDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
Christopher J WhiteDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
NaTasha CarterDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA.
David S HageDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304, USA. Electronic address: dhage1@unl.edu.
University of Nebraska–Lincoln · US

Funding

CHROMATOGRAPHIC AUTOMATION OF IMMUNOASSAYSR01GM044931 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI HAGE, DAVID S · 1998 to 2014
$2.7M
CHROMATOGRAPHIC STUDIES OF FUNCTIONAL PROTEOMICSR01DK069629 · NIDDK · UNIVERSITY OF NEBRASKA LINCOLN · PI HAGE, DAVID S · 2006 to 2017
$1.8M
NIDDK NIH HHS R01 DK069629NIGMS NIH HHS R01 GM044931
6 · The paper itself

Abstract

Affinity chromatography has become an important tool for characterizing biomolecular interactions. The use of affinity microcolumns, which contain immobilized binding agents and have volumes in the mid-to-low microliter range, has received particular attention in recent years. Potential advantages of affinity microcolumns include the many analysis and detection formats that can be used with these columns, as well as the need for only small amounts of supports and immobilized binding agents. This review examines how affinity microcolumns have been used to examine biomolecular interactions. Both capillary-based microcolumns and short microcolumns are considered. The use of affinity microcolumns with zonal elution and frontal analysis methods are discussed. The techniques of peak decay analysis, ultrafast affinity extraction, split-peak analysis, and band-broadening studies are also explored. The principles of these methods are examined and various applications are provided to illustrate the use of these methods with affinity microcolumns. It is shown how these techniques can be utilized to provide information on the binding strength and kinetics of an interaction, as well as on the number and types of binding sites. It is further demonstrated how information on competition or displacement effects can be obtained by these methods.

Indexed as

Chromatography, AffinityHumansKineticsProtein BindingProteinsProteinsAffinity microcolumnsBiointeraction analysisFrontal affinity chromatographyHigh-performance affinity chromatographyUltrafast affinity extractionZonal elution

Identifiers

PMID24572459
PMCPMC4112177
OpenAlexW2128262704

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.