ArticleNature2014
Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1.
Article in Nature, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 181 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
181 citing papers in PubMed, 390 citations in OpenAlex.
- Beyond membrane transport: Transporters as dynamic signal integrators for plant signaling and adaptation.Plant communications · 2026Review
- Beyond prediction: Why experimental structural biology remains essential in plants.Current opinion in plant biology · 2026Review
- The Dual-Function of CtrNAC019-CtrNPF2.1 Module in Salt Tolerance and Nitrogen Use Efficiency Via Enhancing Vacuolar Chloride Sequestration and Nitrate Efflux in Citrus trifoliata.Plant biotechnology journal · 2026Article
- Structure-informed engineering of plant-microbe interactions.The Plant journal : for cell and molecular biology · 2026Review
- Structural basis of glucosinolate recognition and polyspecific transport by the glucosinolate transporter GTR1.The Journal of biological chemistry · 2026Article
- Abiotic Stress Tolerance in Foxtail Millet (Plants (Basel, Switzerland) · 2026Review
- Genetically Encoded Fluorescent Biosensors Enable Noninvasive Real-Time Visualization of Nitrate Dynamics in Intact Living Plants.Biosensors · 2026Article
- Reciprocal co-regulation of nitrate and ammonium transporters is modulated by external pH in Arabidopsis.Journal of experimental botany · 2026Article
- Structural basis of glucosinolate recognition and transport by plant GTR1.Cell discovery · 2026Article
- Tailored for swift recognition: A structural perspective on secondary active plant hormone transporters.The Plant journal : for cell and molecular biology · 2026Review
- Highly efficient production of nitrite and nitrate from air at the gas-water interface of nanobubbles.Science advances · 2026Article
- De Novo Design and Synthesis of Novel Benzoxazinone Derivatives Targeting Dihydroxyacid Dehydratase.Plant biotechnology journal · 2026Article
- Functional characterization of NRT1/PTR FAMILY transporters: looking for a needle in a haystack.The New phytologist · 2026Review
- NRT1.1 as central signaling hub orchestrating plant growth and stress trade-offs.Frontiers in plant science · 2026Review
- An integrative molecular systems approach unravels mechanisms underlying biphasic nitrate uptake by plant nitrate transporter NRT1.1.NPJ systems biology and applications · 2025Article
- Nitrogen Use Efficiency in Agriculture: Integrating Biotechnology, Microbiology, and Novel Delivery Systems for Sustainable Agriculture.Plants (Basel, Switzerland) · 2025Review
- Substrate-based discovery of α-hydroxycarboxylic acid derivatives as potential herbicides targeting dihydroxyacid dehydratase.Nature communications · 2025Article
- Involvement of the miR156/SPLs/NLP7 modules in plant lateral root development and nitrogen uptake.Planta · 2025Article
- Structure and transport mechanism of human riboflavin transporters.Nature communications · 2025Article
- Comparative analysis of STP6 and STP10 unravels molecular selectivity in sugar transport proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
121 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The NRT1/PTR family of proton-coupled transporters are responsible for nitrogen assimilation in eukaryotes and bacteria through the uptake of peptides. However, in most plant species members of this family have evolved to transport nitrate as well as additional secondary metabolites and hormones. In response to falling nitrate levels, NRT1.1 is phosphorylated on an intracellular threonine that switches the transporter from a low-affinity to high-affinity state. Here we present both the apo and nitrate-bound crystal structures of Arabidopsis thaliana NRT1.1, which together with in vitro binding and transport data identify a key role for His 356 in nitrate binding. Our data support a model whereby phosphorylation increases structural flexibility and in turn the rate of transport. Comparison with peptide transporters further reveals how the NRT1/PTR family has evolved to recognize diverse nitrogenous ligands, while maintaining elements of a conserved coupling mechanism within this superfamily of nutrient transporters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.