ArticleNature2014
Crystal structure of the plant dual-affinity nitrate transporter NRT1.1.
Article in Nature, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 157 papers, 1 of them a synthesis that pooled it.
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Who cites it
157 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Responses of rice plant to multiple abiotic stresses revealed by transcriptome meta-analysis and identification of novel genetic factors.Scientific reports · 2025Pooled it
- Beyond membrane transport: Transporters as dynamic signal integrators for plant signaling and adaptation.Plant communications · 2026Review
- Identification and characterization ofHorticulture research · 2026Article
- The Dual-Function of CtrNAC019-CtrNPF2.1 Module in Salt Tolerance and Nitrogen Use Efficiency Via Enhancing Vacuolar Chloride Sequestration and Nitrate Efflux in Citrus trifoliata.Plant biotechnology journal · 2026Article
- Article
- Structural basis of glucosinolate recognition and polyspecific transport by the glucosinolate transporter GTR1.The Journal of biological chemistry · 2026Article
- The NRT1.1-NLP7 Nexus: An Integrative Signaling Nexus from Nitrate Sensing to Systemic Adaptation and Structure-Guided Engineering.Plants (Basel, Switzerland) · 2026Review
- The rice cysteine protease OsEP3A promotes seedling growth and seed development and contains a nitrogen-starvation-responsive sequence.Plant cell reports · 2026Article
- Reciprocal co-regulation of nitrate and ammonium transporters is modulated by external pH in Arabidopsis.Journal of experimental botany · 2026Article
- Structural basis of glucosinolate recognition and transport by plant GTR1.Cell discovery · 2026Article
- Structures of the human glucose-6-phosphate transporter provide insights into its transport cycle and substrate recognition.PLoS biology · 2026Article
- Tailored for swift recognition: A structural perspective on secondary active plant hormone transporters.The Plant journal : for cell and molecular biology · 2026Review
- Physiological and Molecular Mechanisms of Nitrogen Regulation on Grain Quality in Cereal Crops at Later Stages.International journal of molecular sciences · 2026Review
- Symbiosome membrane-localized cationic amino acid transporters support symbiotic nitrogen fixation in Medicago truncatula.Plant communications · 2026Article
- Docking-guided and phosphomimetic rational engineering of glucose-insensitive xylose transporters from Trichoderma reesei.Microbial cell factories · 2026Article
- Soybean Auxin Transporter PIN3 Regulates Nitrate Acquisition to Improve Nitrogen Use and Seed Traits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Functional characterization of NRT1/PTR FAMILY transporters: looking for a needle in a haystack.The New phytologist · 2026Review
- NRT1.1 as central signaling hub orchestrating plant growth and stress trade-offs.Frontiers in plant science · 2026Review
- Nitrogen use efficiency in crops under salt stress: from molecular networks to intelligent breeding.Frontiers in plant science · 2026Review
- High-throughput screening for biostimulant discovery: opportunities to enhance nitrogen use efficiency.Frontiers in plant science · 2026Review
97 more citing papers are in PubMed but not listed here.
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6 authors.
Funding
Abstract
Nitrate is a primary nutrient for plant growth, but its levels in soil can fluctuate by several orders of magnitude. Previous studies have identified Arabidopsis NRT1.1 as a dual-affinity nitrate transporter that can take up nitrate over a wide range of concentrations. The mode of action of NRT1.1 is controlled by phosphorylation of a key residue, Thr 101; however, how this post-translational modification switches the transporter between two affinity states remains unclear. Here we report the crystal structure of unphosphorylated NRT1.1, which reveals an unexpected homodimer in the inward-facing conformation. In this low-affinity state, the Thr 101 phosphorylation site is embedded in a pocket immediately adjacent to the dimer interface, linking the phosphorylation status of the transporter to its oligomeric state. Using a cell-based fluorescence resonance energy transfer assay, we show that functional NRT1.1 dimerizes in the cell membrane and that the phosphomimetic mutation of Thr 101 converts the protein into a monophasic high-affinity transporter by structurally decoupling the dimer. Together with analyses of the substrate transport tunnel, our results establish a phosphorylation-controlled dimerization switch that allows NRT1.1 to uptake nitrate with two distinct affinity modes.
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