ArticleGut2015
The human squamous oesophagus has widespread capacity for clonal expansion from cells at diverse stages of differentiation.
Article in Gut, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Immunohistochemical Study of Diamine Oxidase at the Upper Gastrointestinal Tract in Patients With Gastrointestinal-Mediated Food Allergy.Allergy, asthma & immunology research · 2026Article
- Esophageal ILC2s mediate abnormal epithelial remodeling in eosinophilic esophagitis via Areg-EGFR signaling.Cellular & molecular immunology · 2025Article
- Self-sustaining long-term 3D epithelioid cultures reveal drivers of clonal expansion in esophageal epithelium.Nature genetics · 2024Article
- Non-genetic differences underlie variability in proliferation among esophageal epithelial clones.PLoS computational biology · 2024Article
- Modeling Epithelial Homeostasis and Perturbation in Three-Dimensional Human Esophageal Organoids.Biomolecules · 2024Article
- Identification and characterization of stem cells in mammalian esophageal stratified squamous epithelia.Journal of molecular cell biology · 2022Article
- Mapping the adult human esophagus in vivo and in vitro.Development (Cambridge, England) · 2022Article
- Somatic Mutation: What Shapes the Mutational Landscape of Normal Epithelia?Cancer discovery · 2022Review
- Single-cell transcriptomics provides insights into the origin and microenvironment of human oesophageal high-grade intraepithelial neoplasia.Clinical and translational medicine · 2022Article
- The development and stem cells of the esophagus.Development (Cambridge, England) · 2021Review
- A Scarless Healing Tale: Comparing Homeostasis and Wound Healing of Oral Mucosa With Skin and Oesophagus.Frontiers in cell and developmental biology · 2021Review
- The cellular origins of cancer with particular reference to the gastrointestinal tract.International journal of experimental pathology · 2020Review
- Genetic Mouse Models and Induced Pluripotent Stem Cells for Studying Tracheal-Esophageal Separation and Esophageal Development.Stem cells and development · 2020Article
- Expression of CD117, CD34, and VEGF proteins in progression from endometrial hyperplasia to endometrioid carcinoma.International journal of clinical and experimental pathology · 2020Article
- 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling.Cell stem cell · 2018Article
- Deciphering the cells of origin of squamous cell carcinomas.Nature reviews. Cancer · 2018Review
- Origins of Metaplasia in Barrett's Esophagus: Is this an Esophageal Stem or Progenitor Cell Disease?Digestive diseases and sciences · 2018Review
- Origins of Metaplasia in the Esophagus: Is This a GE Junction Stem Cell Disease?Digestive diseases and sciences · 2018Review
- [Tubular gastric elongation surgery for high esophageal-gastric anastomosis after resection of esophageal cancer: analysis of 5 cases].Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2018Article
- Esophageal Cancer: Genomic and Molecular Characterization, Stem Cell Compartment and Clonal Evolution.Medicines (Basel, Switzerland) · 2017Review
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9 authors.
Funding
Abstract
objectiveKnowledge of the cellular mechanisms involved in homeostasis of human squamous oesophagus in the steady state and following chronic injury is limited. We aimed to better understand these mechanisms by using a functional 3D approach.
designProliferation, mitosis and the expression of progenitor lineage markers were assessed in normal squamous oesophagus from 10 patients by immunofluorescence on 3D epithelial whole mounts. Cells expressing differential levels of epithelial and progenitor markers were isolated using flow cytometry sorting and characterised by qPCR and IF. Their self-renewing potential was investigated by colony forming cells assays and in vitro organotypic culture models.
resultsProliferation and mitotic activity was highest in the interpapillary basal layer and decreased linearly towards the tip of the papilla (p<0.0001). The orientation of mitosis was random throughout the basal layer, and asymmetric divisions were not restricted to specific cell compartments. Cells sorted into distinct populations based on the expression of epithelial and progenitor cell markers (CD34 and EpCAM) showed no difference in self-renewal in 2D culture, either as whole populations or as single cells. In 3D organotypic cultures, all cell subtypes were able to recapitulate the architecture of the tissue of origin and the main factor determining the success of the 3D culture was the number of cells plated, rather than the cell type.
conclusionsOesophageal epithelial cells demonstrate remarkable plasticity for self-renewal. This situation could be viewed as an ex vivo wounding response and is compatible with recent findings in murine models.
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