ArticleIranian journal of basic medical sciences2013
A Consistent PCR-RFLP Assay Based on ITS-2 Ribosomal DNA for Differentiation of Fasciola Species.
Article in Iranian journal of basic medical sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- Discrimination of Hard Ticks by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).International journal of molecular sciences · 2025Article
- Morphometric and Molecular Characterization of Fasciola spp. in Livestock From Northwestern Provinces of Iran.Veterinary medicine and science · 2024Article
- Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran.Veterinary medicine and science · 2023Article
- Identification ofJournal of parasitology research · 2018Article
- Genetic diversity ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2017Article
- Detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2016Article
- Molecular characterization ofVeterinary world · 2016Article
- Epidemiology of Human Fascioliasis and Intestinal Helminthes in Rural Areas of Boyer-Ahmad Township, Southwest Iran; A Population Based Study.Iranian journal of public health · 2015Article
- Genotyping and Phylogenetic Analysis of Fasciola Spp. Isolated from Sheep and Cattle Using PCR-RFLP in Ardabil Province, Northwestern Iran.Iranian journal of public health · 2014Article
- Molecular and Morphological Characterization of Fasciola spp. Isolated from Different Host Species in a Newly Emerging Focus of Human Fascioliasis in Iran.Veterinary medicine international · 2014Article
- Clinical Features, Diagnosis and Management of Patients with Suspicion of Fascioliasis in Kohgiluyeh and Boyer-Ahmad Province, Southwestern Iran.Iranian journal of parasitologyArticle
- Molecular Evaluation of a Case ofIranian journal of parasitologyArticle
- Morphological and Molecular Analysis ofIranian journal of parasitologyArticle
- Performance of a 27 kDa Fasciola hepatica Antigen in the Diagnosis of Human Fascioliasis.Journal of laboratory physiciansArticle
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
OBJECTIVE(S): Fascioliasis is a zoonotic parasitic disease caused by liver fluke species of Fasciola hepatica and Fasciola gigantica. Differentiation of these two species, based on their morphological characteristics, is difficult. The current study aimed to use PCR-RFLP assay to distinguish between F. hepatica and F. gigantica, based on profiles of RFLP, produced by effect of endonucleases on ITS2 of the ribosomal DNA genes from these two species. MATERIALS AND
methodsAdult Fasciola spp. were isolated from bile duct of naturally infected animals. The species of Fasciola were confirmed by sequencing the 505 bp region of the ITS2 gene in the isolates. By running the sequences of the samples in NEBcutter, suitable restriction enzymes (MspI and KpnI) were selected. Eight F. gigantica and eighteen F. hepatica samples were evaluated.
resultsWhile RFLP pattern with MspI produced a profile by which it was difficult to differentiate these two species, KpnI along with MspI, produced a consistent pattern of a 231, 212 and 93 bp fragments in F. hepatica. This pattern was not seen in F. gigantica.
conclusionFindings of this study demonstrated that RFLP with KpnI and MspI produce a suitable pattern which simply differentiates F. hepatica from F. gigantica.
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Identifiers
24570833PMC3933804W2144376231What OpenQuestion holds
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