ArticleChromosoma2014
ELCS in ice: cryo-electron microscopy of nuclear envelope-limited chromatin sheets.
Article in Chromosoma, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Envelope-Limited Chromatin Sheets (ELCS) Formation in The Nuclear Envelope of HL-60/S4 Cells.bioRxiv : the preprint server for biology · 2026Article
- Interphase epichromatin: last refuge for the 30-nm chromatin fiber?Chromosoma · 2021Review
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Non-proliferative neurogenesis in human periodontal ligament stem cells.Scientific reports · 2019Article
- Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes.Chromosoma · 2019Review
- Cryo-ET reveals the macromolecular reorganization ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- The in situ structures of mono-, di-, and trinucleosomes in human heterochromatin.Molecular biology of the cell · 2018Article
- Natural chromatin is heterogeneous and self-associates in vitro.Molecular biology of the cell · 2018Article
- Jörg Langowski: his scientific legacy and the future it promises.BMC biophysics · 2018Article
- Differential staining of peripheral nuclear chromatin with Acridine orange implies an A-form epichromatin conformation of the DNA.Nucleus (Austin, Tex.) · 2018Article
- Analysis of chromatin fibers in Hela cells with electron tomography.Biophysics reports · 2015Article
- Remodeling of nuclear landscapes during human myelopoietic cell differentiation maintains co-aligned active and inactive nuclear compartments.Epigenetics & chromatin · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear envelope-limited chromatin sheets (ELCS) form during excessive interphase nuclear envelope growth in a variety of cells. ELCS appear as extended sheets within the cytoplasm connecting distant nuclear lobes. Cross-section stained images of ELCS, viewed by transmission electron microscopy, resemble a sandwich of apposed nuclear envelopes separated by ∼30 nm, containing a layer of parallel chromatin fibers. In this study, the ultrastructure of ELCS was compared by three different methods: (1) aldehyde fixation/dehydration/plastic embedding/sectioning and staining, (2) high-pressure freezing/freeze substitution into plastic/sectioning and staining, and (3) high-pressure freezing/cryo-sectioning/cryo-electron microscopy. ELCS could be clearly visualized by all three methods and, consequently, must exist in vivo and are not fixation artifacts. The ∼30-nm chromatin fibers could only be observed following aldehyde fixation; none were seen in cryo-sections. Electron microscopic tomography tangential views of aldehyde-fixed ELCS suggested an ordering of the separate chromatin fibers adjacent to the nuclear envelope. Possible mechanisms of this chromatin ordering are discussed.
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Registered trials
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