ArticleMolecular biology reports2014
Molecular cloning and characterization of enhanced disease susceptibility 1 (EDS1) from Gossypium barbadense.
Article in Molecular biology reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Genetic Mapping and QTL Analysis of Stigma Color in Melon (Frontiers in plant science · 2022Article
- Functional validation of pathogenicity genes in rice sheath blight pathogen Rhizoctonia solani by a novel host-induced gene silencing system.Molecular plant pathology · 2021Article
- Biotechnological Potential of LSD1, EDS1, and PAD4 in the Improvement of Crops and Industrial Plants.Plants (Basel, Switzerland) · 2019Review
- CottonFrontiers in plant science · 2019Article
- The dynamic transcriptome and metabolomics profiling in Verticillium dahliae inoculated Arabidopsis thaliana.Scientific reports · 2018Article
- TaEDS1 genes positively regulate resistance to powdery mildew in wheat.Plant molecular biology · 2018Article
- RNA Interference for Functional Genomics and Improvement of Cotton (Gossypium sp.).Frontiers in plant science · 2016Review
- Island Cotton Enhanced Disease Susceptibility 1 Gene Encoding a Lipase-Like Protein Plays a Crucial Role in Response toFrontiers in plant science · 2016Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arabidopsis enhanced disease susceptibility 1 (EDS1) plays an important role in plant defense against biotrophic and necrotrophic pathogens. The necrotrophic pathogen Verticillium dahliae infection of Gossypium barbadense could lead to Verticillium wilt which seriously reduces the cotton production. Here, we cloned and characterized a G. barbadense homolog of EDS1, designated as GbEDS1. The full-length cDNA of the GbEDS1 gene was obtained by the technique of rapid-amplification of cDNA ends. The open reading frame of the GbEDS1 gene was 1,647 bp long and encoded a protein of 548 amino acids residues. Comparison of the cDNA and genomic DNA sequence of GbEDS1 indicated that this gene contained a single intron and two exons. Like other EDS1s, GbEDS1 contained a conserved N-terminal lipase domain and an EDS1-specific KNEDT motif. Subcellular localization assay revealed that GbEDS1-green fluorescence protein fusion protein was localized in both cytosol and nucleus. Interestingly, the transcript levels of GbEDS1 were dramatically increased in response to pathogen V. dahliae infection. To investigate the role of GbEDS1 in plant resistance against V. dahliae, a conserved fragment derived from GbEDS1 was used to knockdown the endogenous EDS1 in Nicotiana benthamiana by heterologous virus-induced gene silencing. Our data showed that silencing of NbEDS1 resulted in increased susceptibility to V. dahliae infection in N. benthamiana, suggesting a possible involvement of the novelly isolated GbEDS1 in the regulation of plant defense against V. dahliae.
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