ArticleNature communications2014
SILAC-based proteomic quantification of chemoattractant-induced cytoskeleton dynamics on a second to minute timescale.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
- The Proteome ofProteomes · 2026Article
- Leep2A and Leep2B function as a RasGAP complex to regulate macropinosome formation.The Journal of cell biology · 2024Article
- Proteomic Analysis of Dictyostelium discoideum by Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Gradients of PI(4,5)PFrontiers in cell and developmental biology · 2022Article
- Moving the Research Forward: The Best of British Biology Using the Tractable Model SystemCells · 2021Review
- Suppression of a core metabolic enzyme dihydrolipoamide dehydrogenase (Genes & diseases · 2021Article
- Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology.BMC biology · 2021Article
- Leep1 interacts with PIP3 and the Scar/WAVE complex to regulate cell migration and macropinocytosis.The Journal of cell biology · 2021Article
- Article
- The Atypical MAP Kinase ErkB Transmits Distinct Chemotactic Signals through a Core Signaling Module.Developmental cell · 2019Article
- Fam49/CYRI interacts with Rac1 and locally suppresses protrusions.Nature cell biology · 2018Article
- KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition.Oncogene · 2017Article
- Thematic Minireview Series: The State of the Cytoskeleton in 2015.The Journal of biological chemistry · 2015Review
- The Dictyostelium prestalk inducer differentiation-inducing factor-1 (DIF-1) triggers unexpectedly complex global phosphorylation changes.Molecular biology of the cell · 2015Article
- The emergence of proteome-wide technologies: systematic analysis of proteins comes of age.Nature reviews. Molecular cell biology · 2014Review
- Systems Biology Approaches Applied to Regenerative Medicine.Current pathobiology reportsReview
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Cytoskeletal dynamics during cell behaviours ranging from endocytosis and exocytosis to cell division and movement is controlled by a complex network of signalling pathways, the full details of which are as yet unresolved. Here we show that SILAC-based proteomic methods can be used to characterize the rapid chemoattractant-induced dynamic changes in the actin-myosin cytoskeleton and regulatory elements on a proteome-wide scale with a second to minute timescale resolution. This approach provides novel insights in the ensemble kinetics of key cytoskeletal constituents and association of known and novel identified binding proteins. We validate the proteomic data by detailed microscopy-based analysis of in vivo translocation dynamics for key signalling factors. This rapid large-scale proteomic approach may be applied to other situations where highly dynamic changes in complex cellular compartments are expected to play a key role.
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Registered trials
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