ArticleThe EMBO journal2014
Cell segregation in the vertebrate hindbrain relies on actomyosin cables located at the interhombomeric boundaries.
Article in The EMBO journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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59 citing papers in PubMed, 123 citations in OpenAlex.
- Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.Journal of biomedical optics · 2026Article
- The Role of Eph/Ephrin-Driven Actomyosin Contractility in Promoting Cell Segregation and Boundary Formation.International journal of molecular sciences · 2026Review
- Cell divisions both challenge and refine tissue boundaries in the Drosophila embryo.Development (Cambridge, England) · 2026Article
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- Her9 controls the stemness properties of hindbrain boundary cells.Development (Cambridge, England) · 2025Article
- Spatial Transcriptome and Single Nucleus Transcriptome Sequencing Reveals Tetrahydroxy Stilbene Glucoside Promotes Ovarian Organoids Development Through the Vegfa-Ephb2 Pair.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Differences in boundary behavior in the 3D vertex and Voronoi models.PLoS computational biology · 2024Article
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- Preferential recruitment and stabilization of Myosin II at compartment boundaries in Drosophila.Journal of cell science · 2023Article
- Homeotic compartment curvature and tension control spatiotemporal folding dynamics.Nature communications · 2023Article
- Hindbrain rhombomere centers harbor a heterogenous population of dividing progenitors which rely on Notch signaling.Frontiers in cell and developmental biology · 2023Article
- Different temporal requirements for tartan and wingless in the formation of contractile interfaces at compartmental boundaries.Development (Cambridge, England) · 2022Article
- Adhesion-Based Self-Organization in Tissue Patterning.Annual review of cell and developmental biology · 2022Review
- Autocrine/Paracrine Slit-Robo Signaling Controls Optic Lobe Development inFrontiers in cell and developmental biology · 2022Article
- Segmentation and patterning of the vertebrate hindbrain.Development (Cambridge, England) · 2021Review
- EPH/EPHRIN regulates cellular organization by actomyosin contractility effects on cell contacts.The Journal of cell biology · 2021Article
- Actin-based force generation and cell adhesion in tissue morphogenesis.Current biology : CB · 2021Review
- Arp2/3-dependent mechanical control of morphogenetic robustness in an inherently challenging environment.Developmental cell · 2021Article
- Article
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Segregating cells into compartments during embryonic development is essential for growth and pattern formation. Physical mechanisms shaping compartment boundaries were recently explored in Drosophila, where actomyosin-based barriers were revealed to be important for keeping cells apart. In vertebrates, interhombomeric boundaries are straight interfaces, which often serve as signaling centers that pattern the surrounding tissue. Here, we demonstrate that in the hindbrain of zebrafish embryos cell sorting sharpens the molecular boundaries and, once borders are straight, actomyosin barriers are key to keeping rhombomeric cells segregated. Actomyosin cytoskeletal components are enriched at interhombomeric boundaries, forming cable-like structures in the apical side of the neuroepithelial cells by the time morphological boundaries are visible. When myosin II function is inhibited, cable structures do not form, leading to rhombomeric cell mixing. Downregulation of EphA4a compromises actomyosin cables and cells with different rhombomeric identity intermingle, and the phenotype is rescued enhancing myosin II activity. Moreover, enrichment of actomyosin structures is obtained when EphA4 is ectopically expressed in even-numbered rhombomeres. These findings suggest that mechanical barriers act downstream of EphA/ephrin signaling to segregate cells from different rhombomeres.
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