ArticleG3 (Bethesda, Md.)2014
Extensive cotransformation of natural variation into chromosomes of naturally competent Haemophilus influenzae.
Article in G3 (Bethesda, Md.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Emergence, evolution, and global dissemination of antimicrobial resistance: A One Health review.Archives of microbiology · 2026Review
- Review
- Introgression impacts the evolution of bacteria, but species borders are rarely fuzzy.Nature communications · 2025Article
- Manipulation of natural transformation by AbaR-type islands promotes fixation of antibiotic resistance inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Genome-wide transformation reveals extensive exchange across closely related Bacillus species.Nucleic acids research · 2023Article
- High Prevalence of Group III-Like Mutations Among BLPACR and First Report ofInfection and drug resistance · 2023Article
- Article
- Repeat sequences limit the effectiveness of lateral gene transfer and favored the evolution of meiotic sex in early eukaryotes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Protoplast fusion in Bacillus species produces frequent, unbiased, genome-wide homologous recombination.Nucleic acids research · 2022Article
- Recombination resolves the cost of horizontal gene transfer in experimental populations ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Challenges Faced by Highly Polyploid Bacteria with Limits on DNA Inheritance.Genome biology and evolution · 2021Review
- Adaptive evolution of hybrid bacteria by horizontal gene transfer.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Customized optical mapping by CRISPR-Cas9 mediated DNA labeling with multiple sgRNAs.Nucleic acids research · 2021Article
- Learning from -omics strategies applied to uncoverComputational and structural biotechnology journal · 2021Review
- Experimental Evolution ofGenetics · 2020Article
- Genome expansion in early eukaryotes drove the transition from lateral gene transfer to meiotic sex.eLife · 2020Article
- Multiplex gene transfer by genetic transformation between isolated S. pneumoniae cells confined in microfluidic droplets.Integrative biology : quantitative biosciences from nano to macro · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Naturally competent bacterial species actively take up environmental DNA and can incorporate it into their chromosomes by homologous recombination. This can bring genetic variation from environmental DNA to recipient chromosomes, often in multiple long "donor" segments. Here, we report the results of genome sequencing 96 colonies of a laboratory Haemophilus influenzae strain, which had been experimentally transformed by DNA from a diverged clinical isolate. Donor segments averaged 6.9 kb (spanning several genes) and were clustered into recombination tracts of ~19.5 kb. Individual colonies had replaced from 0.1 to 3.2% of their chromosomes, and ~1/3 of all donor-specific single-nucleotide variants were present in at least one recombinant. We found that nucleotide divergence did not obviously limit the locations of recombination tracts, although there were small but significant reductions in divergence at recombination breakpoints. Although indels occasionally transformed as parts of longer recombination tracts, they were common at breakpoints, suggesting that indels typically block progression of strand exchange. Some colonies had recombination tracts in which variant positions contained mixtures of both donor and recipient alleles. These tracts were clustered around the origin of replication and were interpreted as the result of heteroduplex segregation in the original transformed cell. Finally, a pilot experiment demonstrated the utility of natural transformation for genetically dissecting natural phenotypic variation. We discuss our results in the context of the potential to merge experimental and population genetic approaches, giving a more holistic understanding of bacterial gene transfer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.