ArticleJournal of membrane science2014
Microfabrication of High-Resolution Porous Membranes for Cell Culture.
Article in Journal of membrane science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Engineering biomimetic tissue barrier models on chips: From design and fabrication to applications in disease modeling and drug screening.Biomaterials · 2026Review
- Fabrication of Biomimetic Cell Culture Membranes Using Robust and Reusable Nickel Micropillar Molds.Biochip journal · 2025Article
- Fabrication Method for Shape-Controlled 3D Tissue Using High-Porosity Porous Structure.Bioengineering (Basel, Switzerland) · 2024Article
- Integration of Extracellular Matrices into Organ-on-Chip Systems.Advanced healthcare materials · 2023Review
- A novel 4-cell in-vitro blood-brain barrier model and its characterization by confocal microscopy and TEER measurement.Journal of neuroscience methods · 2023Article
- Optimization of Parylene C and Parylene N thin films for use in cellular co-culture and tissue barrier models.Scientific reports · 2023Article
- Immunohistological responses in mice implanted with Parylene HT - ITO ECoG devices.Frontiers in neuroscience · 2023Article
- Polymeric and biological membranes for organ-on-a-chip devices.Microsystems & nanoengineering · 2023Review
- Femtosecond Laser Fabrication of Microporous Membranes for Biological Applications.Micromachines · 2022Article
- Mimicking the Natural Basement Membrane for Advanced Tissue Engineering.Biomacromolecules · 2022Review
- Electrohydrodynamic Jet-Printed Ultrathin Polycaprolactone Scaffolds Mimicking Bruch's Membrane for Retinal Pigment Epithelial Tissue Engineering.International journal of bioprinting · 2022Article
- Glomerulus-on-a-Chip: Current Insights and Future Potential Towards Recapitulating Selectively Permeable Filtration Systems.International journal of nephrology and renovascular disease · 2022Review
- Modelling of Stem Cells Microenvironment Using Carbon-Based Scaffold for Tissue Engineering Application-A Review.Polymers · 2021Review
- Development of Real-Time Transendothelial Electrical Resistance Monitoring for an In Vitro Blood-Brain Barrier System.Micromachines · 2020Article
- Robust and Gradient Thickness Porous Membranes forAdvanced materials technologies · 2020Article
- Use of Nanosphere Self-Assembly to Pattern Nanoporous Membranes for the Study of Extracellular Vesicles.Nanoscale advances · 2020Article
- Rapid Fabrication of Membrane-Integrated Thermoplastic Elastomer Microfluidic Devices.Micromachines · 2020Article
- Ultrathin Silicon Membranes forACS nano · 2020Article
- Review
- Micropatterning Method for Porous Materials Using the Difference of the Glass Transition Temperature between Exposed and Unexposed Areas of a Thick-Photoresist.Micromachines · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Microporous membranes are widely utilized in cell biology to study cell-cell signaling and cell migration. However, the thickness and low porosity of commercial track-etched membranes limit the quality of cell imaging and the degree of cell-cell contact that can be achieved on such devices. We employ photolithography-based microfabrication to achieve porous membranes with pore diameter as small as 0.9 μm, up to 40% porosity, and less than 5% variation in pore size. Through the use of a soap release layer, membranes as thin as 1 μm can be achieved. The thin membranes minimally disrupt contrast enhancement optics, thus allowing good quality imaging of unlabeled cells under white light, unlike commercial membranes. In addition, the polymer membrane materials display low autofluorescence even after patterning, facilitating high quality fluorescence microscopy. Finally, confocal imaging suggests that substantial cell-cell contact is possible through the pores of these thin membranes. This membrane technology can enhance existing uses of porous membranes in cell biology as well as enable new types of experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.