ArticleThe Journal of experimental medicine2014
Asymmetry in skeletal distribution of mouse hematopoietic stem cell clones and their equilibration by mobilizing cytokines.
Article in The Journal of experimental medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 60 citations in OpenAlex.
- Deciphering the Complexities of Adult Human Steady State and Stress-Induced Hematopoiesis: Progress and Challenges.International journal of molecular sciences · 2025Review
- Panhematopoietic RNA barcoding enables kinetic measurements of nucleate and anucleate lineages and the activation of myeloid clones following acute platelet depletion.Genome biology · 2023Article
- The Reconstitution Dynamics of Cultivated Hematopoietic Stem Cells and Progenitors Is Independent of Age.International journal of molecular sciences · 2022Article
- Measures of Clonal Hematopoiesis: Are We Missing Something?Frontiers in medicine · 2022Article
- Clonal tracking of haematopoietic cells: insights and clinical implications.British journal of haematology · 2021Review
- DNA Barcoding in Nonhuman Primates Reveals Important Limitations in Retrovirus Integration Site Analysis.Molecular therapy. Methods & clinical development · 2020Article
- Development of anFuture science OA · 2019Article
- MiR-125a enhances self-renewal, lifespan, and migration of murine hematopoietic stem and progenitor cell clones.Scientific reports · 2019Article
- Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation.The Journal of experimental medicine · 2018Article
- Limitations and challenges of genetic barcode quantification.Scientific reports · 2017Article
- A Lentiviral Fluorescent Genetic Barcoding System for Flow Cytometry-Based Multiplex Tracking.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells.Cell · 2017Article
- Reproducibility of Illumina platform deep sequencing errors allows accurate determination of DNA barcodes in cells.BMC bioinformatics · 2016Article
- Inflamm-Aging of Hematopoiesis, Hematopoietic Stem Cells, and the Bone Marrow Microenvironment.Frontiers in immunology · 2016Review
- Development of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Regulation of hematopoietic stem cell aging by the small RhoGTPase Cdc42.Experimental cell research · 2014Review
- Modeling promising nonmyeloablative conditioning regimens in nonhuman primates.Human gene therapy · 2014Article
- Aging of the hematopoietic stem cells niche.International journal of hematology · 2014Review
- Niche displacement of human leukemic stem cells uniquely allows their competitive replacement with healthy HSPCs.The Journal of experimental medicine · 2014Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hematopoietic stem cells (HSCs) are able to migrate through the blood stream and engraft bone marrow (BM) niches. These features are key factors for successful stem cell transplantations that are used in cancer patients and in gene therapy protocols. It is unknown to what extent transplanted HSCs distribute throughout different anatomical niches in the BM and whether this changes with age. Here we determine the degree of hematopoietic migration at a clonal level by transplanting individual young and aged mouse HSCs labeled with barcoded viral vector, followed by assessing the skeletal distribution of hundreds of HSC clones. We detected highly skewed representation of individual clones in different bones at least 11 mo after transplantation. Importantly, a single challenge with the clinically relevant mobilizing agent granulocyte colony-stimulating factor (G-CSF) caused rapid redistribution of HSCs across the skeletal compartments. Old and young HSC clones showed a similar level of migratory behavior. Clonal make-up of blood of secondary recipients recapitulates the barcode composition of HSCs in the bone of origin. These data demonstrate a previously unanticipated high skeletal disequilibrium of the clonal composition of HSC pool long-term after transplantation. Our findings have important implications for experimental and clinical and stem cell transplantation protocols.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.