ArticleProceedings of the National Academy of Sciences of the United States of America2014
A propagating ATPase gradient drives transport of surface-confined cellular cargo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed, 182 citations in OpenAlex.
- Molecular Insights on the Assembling of ParB1 and ParB1-ACS bio & med chem Au · 2026Article
- The kinetics and mobility of a ParA ATPase drive carboxysome distribution inbioRxiv : the preprint server for biology · 2026Article
- Brownian ratchet mechanisms for carboxysome positioning in bacteria.Current opinion in microbiology · 2025Review
- Expanding the diversity of bacterial DNA partitioning: A CTP-independent ParABProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mutational analysis of the F plasmid partitioning protein ParA reveals residues required for oligomerization and plasmid maintenance.Nucleic acids research · 2025Article
- Significance of the CTP-binding motif for the interactions of S. coelicolor ParB with DNA, chromosome segregation, and sporogenic hyphal growth.Nucleic acids research · 2025Article
- Acoustofluidic spin control for 3D particle manipulation in droplets.Science advances · 2025Article
- DNA supercoiling enhances DNA condensation by ParB proteins.Nucleic acids research · 2024Article
- Coordinating plasmid partition with bacterial chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Plasmid partitioning driven by collective migration of ParA between nucleoid lobes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Motility of an autonomous protein-based artificial motor that operates via a burnt-bridge principle.Nature communications · 2024Article
- Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.FEMS microbiology reviews · 2024Review
- TipN's involvement with centromere segregation in Caulobacter crescentus.bioRxiv : the preprint server for biology · 2023Article
- DNA Segregation in Enterobacteria.EcoSal Plus · 2023Review
- VirB, a key transcriptional regulator ofmBio · 2023Article
- Cell cycle-dependent organization of a bacterial centromere through multi-layered regulation of the ParABS system.PLoS genetics · 2023Article
- Kinetic principles of ParA2-ATP cycling guide dynamic subcellular localizations in Vibrio cholerae.Nucleic acids research · 2023Article
- VirB, a key transcriptional regulator ofbioRxiv : the preprint server for biology · 2023Article
- A new role for monomeric ParA/Soj in chromosome dynamics in Bacillus subtilis.MicrobiologyOpen · 2023Article
- Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirochete.Nature communications · 2022Article
34 more citing papers are in PubMed but not listed here.
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3 authors at 2 institutions in 1 country.
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Abstract
The faithful segregation of duplicated genetic material into daughter cells is critical to all organisms. In many bacteria, the segregation of chromosomes involves transport of "centromere-like" loci over the main body of the chromosome, the nucleoid, mediated by a two-protein partition system: a nonspecific DNA-binding ATPase, ParA, and an ATPase stimulator, ParB, which binds to the centromere-like loci. These systems have previously been proposed to function through a filament-based mechanism, analogous to actin- or microtubule-based movement. Here, we reconstituted the F-plasmid partition system using a DNA-carpeted flow cell as an artificial nucleoid surface and magnetic beads coated with plasmid partition complexes as surface-confined cargo. This minimal system recapitulated directed cargo motion driven by a surface ATPase gradient that propagated with the cargo. The dynamics are consistent with a diffusion-ratchet model, whereby the cargo dynamically establishes, and interacts with, a concentration gradient of the ATPase. A chemophoresis force ensues as the cargo perpetually chases the ATPase gradient, allowing the cargo to essentially "surf" the nucleoid on a continuously traveling wave of the ATPase. Demonstration of this non-filament-based motility mechanism in a biological context establishes a distinct class of motor system used for the transport and positioning of large cellular cargo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.