ArticleProceedings of the National Academy of Sciences of the United States of America2014
Self-assembly of the general membrane-remodeling protein PVAP into sevenfold virus-associated pyramids.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Insights into the spool-like architecture and infection strategy of an enveloped archaeal virus.Science advances · 2025Article
- Diversity and evolution of prokaryotic viral lytic proteins.The ISME journal · 2025Article
- The intricate organizational strategy of nucleus-forming phages.Current opinion in microbiology · 2024Review
- Archaeal virus entry and egress.microLife · 2024Review
- Viruses of theFrontiers in microbiology · 2023Review
- Forty years in cryoEM of membrane proteins.Microscopy (Oxford, England) · 2022Article
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- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- A filamentous archaeal virus is enveloped inside the cell and released through pyramidal portals.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The switch complex ArlCDE connects the chemotaxis system and the archaellum.Molecular microbiology · 2020Article
- The intriguing world of archaeal viruses.PLoS pathogens · 2020Article
- Architecture and modular assembly ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- New archaeal viruses discovered by metagenomic analysis of viral communities in enrichment cultures.Environmental microbiology · 2019Article
- Foreword to 'Multiscale structural biology: biophysical principles and mechanisms underlying the action of bio-nanomachines', a special issue in Honour of Fumio Arisaka's 70th birthday.Biophysical reviews · 2018Article
- Structure and assembly mechanism of virus-associated pyramids.Biophysical reviews · 2018Review
- Novel Sulfolobus Virus with an Exceptional Capsid Architecture.Journal of virology · 2018Article
- The enigmatic archaeal virosphere.Nature reviews. Microbiology · 2017Review
- Article
- Structure andeLife · 2017Article
- Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses have developed a wide range of strategies to escape from the host cells in which they replicate. For egress some archaeal viruses use a pyramidal structure with sevenfold rotational symmetry. Virus-associated pyramids (VAPs) assemble in the host cell membrane from the virus-encoded protein PVAP and open at the end of the infection cycle. We characterize this unusual supramolecular assembly using a combination of genetic, biochemical, and electron microscopic techniques. By whole-cell electron cryotomography, we monitored morphological changes in virus-infected host cells. Subtomogram averaging reveals the VAP structure. By heterologous expression of PVAP in cells from all three domains of life, we demonstrate that the protein integrates indiscriminately into virtually any biological membrane, where it forms sevenfold pyramids. We identify the protein domains essential for VAP formation in PVAP truncation mutants by their ability to remodel the cell membrane. Self-assembly of PVAP into pyramids requires at least two different, in-plane and out-of-plane, protein interactions. Our findings allow us to propose a model describing how PVAP arranges to form sevenfold pyramids and suggest how this small, robust protein may be used as a general membrane-remodeling system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.