ArticleMolecular and cellular biology2014
Simultaneous gene editing by injection of mRNAs encoding transcription activator-like effector nucleases into mouse zygotes.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- Anephrogenic phenotype induced by SALL1 gene knockout in pigs.Scientific reports · 2019Article
- How mRNA therapeutics are entering the monoclonal antibody field.Journal of translational medicine · 2019Review
- Gene editing of the multi-copy H2A.B gene and its importance for fertility.Genome biology · 2019Article
- Mouse medulloblastoma driven by CRISPR activation of cellular Myc.Scientific reports · 2018Article
- Establishment of a tagged variant of Lgr4 receptor suitable for functional and expression studies in the mouse.Transgenic research · 2017Article
- Small mitochondrial Arf (smArf) protein corrects p53-independent developmental defects ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing.Nature communications · 2016Article
- Generation of outbred Ace2 knockout mice by RNA transfection of TALENs displaying colitis reminiscent pathophysiology and inflammation.Transgenic research · 2015Article
- TALEN-mediated genome engineering to generate targeted mice.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- TALEN-mediated genetic tailoring as a tool to analyze the function of acquired mutations in multiple myeloma cells.Blood cancer journal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Injection of transcription activator-like effector nucleases (TALEN) mRNAs into mouse zygotes transferred into foster mothers efficiently generated founder mice with heritable mutations in targeted genes. Immunofluorescence visualization of phosphorylated histone 2A (γH2AX) combined with fluorescence in situ hybridization revealed that TALEN pairs targeting the Agouti locus induced site-directed DNA breaks in zygotes within 6 h of injection, an activity that continued at reduced efficiency in two-cell embryos. TALEN-Agouti mRNAs injected into zygotes of brown FvB × C57BL/6 hybrid mice generated completely black pups, confirming that mutations were induced prior to, and/or early after, cell division. Founder mice, many of which were mosaic, transmitted altered Agouti alleles to F1 pups to yield an allelic series of mutant strains. Although mutations were targeted to "spacer" sequences flanked by TALEN binding sites, larger deletions that extended beyond the TALEN-binding sequences were also detected and were similarly inherited through the germ line. Zygotic coinjection of TALEN mRNAs directed to the Agouti, miR-205, and the Arf tumor suppressor loci yielded pups containing frequent and heritable mutations of two or three genes. Simultaneous gene editing in zygotes affords an efficient approach for producing mice with compound mutant phenotypes, bypassing constraints of conventional mouse knockout technology in embryonic stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.