ArticlePlant physiology2014
The Arabidopsis CALLOSE DEFECTIVE MICROSPORE1 gene is required for male fertility through regulating callose metabolism during microsporogenesis.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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53 citing papers in PubMed, 106 citations in OpenAlex.
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- Advances in plant male sterility for hybrid seed production: an overview of conditional nuclear male sterile lines and biotechnology-based male sterile systems.Frontiers in plant science · 2025Review
- Integrating transcriptomic and proteomic analyses reveals impaired carbohydrate metabolism in tobacco cytoplasmic male sterility.Frontiers in plant science · 2025Article
- Genome-Wide Identification of GH17s Family Genes and Biological Function Analysis of SlA6 in Tomato.Plants (Basel, Switzerland) · 2024Article
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- Transcription Factors and Their Regulatory Roles in the Male Gametophyte Development of Flowering Plants.International journal of molecular sciences · 2024Review
- Triphasic regulation of ZmMs13 encoding an ABCG transporter is sequentially required for callose dissolution, pollen exine and anther cuticle formation in maize.Journal of advanced research · 2023Article
- The transcription factor PbrbZIP52 positively affects pear pollen tube longevity by promoting callose synthesis.Plant physiology · 2023Article
- Review
- Cytosolic phosphoglucose isomerase is essential for microsporogenesis and embryogenesis in Arabidopsis.Plant physiology · 2023Article
- Heat stress during male meiosis impairs cytoskeletal organization, spindle assembly and tapetum degeneration in wheat.Frontiers in plant science · 2023Article
- Hormone Regulation of CCCH Zinc Finger Proteins in Plants.International journal of molecular sciences · 2022Review
- The CCCH zinc finger protein C3H15 negatively regulates cell elongation by inhibiting brassinosteroid signaling.Plant physiology · 2022Article
- OsMYB103 is essential for tapetum degradation in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- MAP3Kε1/2 Interact with MOB1A/1B and Play Important Roles in Control of Pollen Germination through Crosstalk with JA Signaling in Arabidopsis.International journal of molecular sciences · 2022Article
- Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species.Frontiers in plant science · 2022Article
- Arabidopsis Novel Microgametophyte Defective Mutant 1 Is Required for Pollen ViabilityFrontiers in plant science · 2022Article
- MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean.Science China. Life sciences · 2021Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During angiosperm microsporogenesis, callose serves as a temporary wall to separate microsporocytes and newly formed microspores in the tetrad. Abnormal callose deposition and dissolution can lead to degeneration of developing microspores. However, genes and their regulation in callose metabolism during microsporogenesis still remain largely unclear. Here, we demonstrated that the Arabidopsis (Arabidopsis thaliana) CALLOSE DEFECTIVE MICROSPORE1 (CDM1) gene, encoding a tandem CCCH-type zinc finger protein, plays an important role in regulation of callose metabolism in male meiocytes and in integrity of newly formed microspores. First, quantitative reverse transcription PCR and in situ hybridization analyses showed that the CDM1 gene was highly expressed in meiocytes and the tapetum from anther stages 4 to 7. In addition, a transfer DNA insertional cdm1 mutant was completely male sterile. Moreover, light microscopy of anther sections revealed that microspores in the mutant anther were initiated, and then degenerated soon afterward with callose deposition defects, eventually leading to male sterility. Furthermore, transmission electron microscopy demonstrated that pollen exine formation was severely affected in the cdm1 mutant. Finally, we found that the cdm1 mutation affected the expression of callose synthesis genes (CALLOSE SYNTHASE5 and CALLOSE SYNTHASE12) and potential callase-related genes (A6 and MYB80), as well as three other putative β-1,3-glucanase genes. Therefore, we propose that the CDM1 gene regulates callose metabolism during microsporogenesis, thereby promoting Arabidopsis male fertility.
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