ArticleThe EMBO journal2014
Origin plasticity during budding yeast DNA replication in vitro.
Article in The EMBO journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 62 citations in OpenAlex.
- Leishmania major chromosomes are replicated from a single high-efficiency locus supplemented by thousands of lower efficiency initiation events.Cell reports · 2025Article
- G-quadruplex-stalled eukaryotic replisome structure reveals helical inchworm DNA translocation.Science (New York, N.Y.) · 2025Article
- Origins of DNA replication in eukaryotes.Molecular cell · 2023Review
- Mobile origin-licensing factors confer resistance to conflicts with RNA polymerase.Cell reports · 2022Article
- Article
- Preventing excess replication origin activation to ensure genome stability.Trends in genetics : TIG · 2022Review
- Article
- Positive and Negative Regulation of DNA Replication Initiation.Trends in genetics : TIG · 2020Review
- Cell-free biogenesis of bacterial division proto-rings that can constrict liposomes.Communications biology · 2020Article
- INO80C Remodeler Maintains Genomic Stability by Preventing Promiscuous Transcription at Replication Origins.Cell reports · 2020Article
- Article
- FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing.Genome biology · 2020Article
- Rad53 limits CMG helicase uncoupling from DNA synthesis at replication forks.Nature structural & molecular biology · 2020Article
- Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads.Nature methods · 2019Article
- Transcription shapes DNA replication initiation and termination in human cells.Nature structural & molecular biology · 2019Article
- DNA replication through a chromatin environment.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Dormant origins as a built-in safeguard in eukaryotic DNA replication against genome instability and disease development.DNA repair · 2017Review
- Single-molecule analysis reveals that DNA replication dynamics vary across the course of schizogony in the malaria parasite Plasmodium falciparum.Scientific reports · 2017Article
- Article
- Chromatin Controls DNA Replication Origin Selection, Lagging-Strand Synthesis, and Replication Fork Rates.Molecular cell · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The separation of DNA replication origin licensing and activation in the cell cycle is essential for genome stability across generations in eukaryotic cells. Pre-replicative complexes (pre-RCs) license origins by loading Mcm2-7 complexes in inactive form around DNA. During origin firing in S phase, replisomes assemble around the activated Mcm2-7 DNA helicase. Budding yeast pre-RCs have previously been reconstituted in vitro with purified proteins. Here, we show that reconstituted pre-RCs support replication of plasmid DNA in yeast cell extracts in a reaction that exhibits hallmarks of cellular replication initiation. Plasmid replication in vitro results in the generation of covalently closed circular daughter molecules, indicating that the system recapitulates the initiation, elongation, and termination stages of DNA replication. Unexpectedly, yeast origin DNA is not strictly required for DNA replication in vitro, as heterologous DNA sequences could support replication of plasmid molecules. Our findings support the notion that epigenetic mechanisms are important for determining replication origin sites in budding yeast, highlighting mechanistic principles of replication origin specification that are common among eukaryotes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.