ArticleJournal of industrial microbiology & biotechnology2014
Physiological characterization of ATP-citrate lyase in Aspergillus niger.
Article in Journal of industrial microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Microbial cell factories for gamma-linolenic acid biosynthesis: metabolic engineering, downstream processing, and future perspectives.Bioresources and bioprocessing · 2026Review
- The role of ATP citrate lyase, phosphoketolase, and malic enzyme in oleaginous Rhodotorula toruloides.Applied microbiology and biotechnology · 2025Article
- Versatile filamentous fungal host highly-producing heterologous natural products developed by genome editing-mediated engineering of multiple metabolic pathways.Communications biology · 2024Article
- Establishing a one-step marker-free CRISPR/Cas9 system for industrial Aspergillus niger using counter-selectable marker Ang-ace2.Biotechnology letters · 2023Article
- The sensor histidine kinase (SLN1) and acetyl-CoA carboxylase (ACC1) coordinately regulate the response ofApplied and environmental microbiology · 2023Article
- Engineering a Phosphoketolase Pathway to Supplement Cytosolic Acetyl-CoA inJournal of fungi (Basel, Switzerland) · 2023Article
- Identification of chitin synthase activator in Aspergillus niger and its application in citric acid fermentation.Applied microbiology and biotechnology · 2022Article
- Identification and genetic characterization of mitochondrial citrate transporters inFrontiers in microbiology · 2022Article
- Identification and importance of mitochondrial citrate carriers and ATP citrate lyase for glycolipid production in Starmerella bombicola.Applied microbiology and biotechnology · 2020Article
- Article
- Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase inBiotechnology for biofuels · 2020Article
- Biosynthesis of Alkylcitric Acids inFrontiers in microbiology · 2020Article
- Mitochondrial Citrate Transporters CtpA and YhmA Are Required for Extracellular Citric Acid Accumulation and Contribute to Cytosolic Acetyl Coenzyme A Generation in Aspergillus luchuensis mut.Applied and environmental microbiology · 2019Article
- Citric acid and itaconic acid accumulation: variations of the same story?Applied microbiology and biotechnology · 2019Review
- Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era.Microbial cell factories · 2019Review
- Highly efficient improvement of3 Biotech · 2018Article
- Metabolic differences of industrial acarbose-producing Actinoplanes sp. A56 under various osmolality levels.World journal of microbiology & biotechnology · 2016Article
- Endogenous cross-talk of fungal metabolites.Frontiers in microbiology · 2014Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetyl-CoA, an important molecule in cellular metabolism, is generated in multiple subcellular compartments and mainly used for energy production, biosynthesis of a diverse set of molecules, and protein acetylation. In eukaryotes, cytosolic acetyl-CoA is derived mainly from the conversion of citrate and CoA by ATP-citrate lyase. Here, we describe the targeted deletions of acl1 and acl2, two tandem divergently transcribed genes encoding subunits of ATP-citrate lyase in Aspergillus niger. We show that loss of acl1 or/and acl2 results in a significant decrease of acetyl-CoA and citric acid levels in these mutants, concomitant with diminished vegetative growth, decreased pigmentation, reduced asexual conidiogenesis, and delayed conidial germination. Exogenous addition of acetate repaired the defects of acl-deficient strains in growth and conidial germination but not pigmentation and conidiogenesis. We demonstrate that both Acl1 and Acl2 subunits are required to form a functional ATP-citrate lyase in A. niger. First, deletion of acl1 or/and acl2 resulted in similar defects in growth and development. Second, enzyme activity assays revealed that loss of either acl1 or acl2 gene resulted in loss of ATP-citrate lyase activity. Third, in vitro enzyme assays using bacterially expressed 6His-tagged Acl protein revealed that only the complex of Acl1 and Acl2 showed ATP-citrate lyase activity, no enzyme activities were detected with the individual protein. Fourth, EGFP-Acl1 and mCherry-Acl2 proteins were co-localized in the cytosol. Thus, acl1 and acl2 coordinately modulate the cytoplasmic acetyl-CoA levels to regulate growth, development, and citric acid synthesis in A. niger.
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