ArticleEcotoxicology (London, England)2014
Identification of suitable qPCR reference genes in leaves of Brassica oleracea under abiotic stresses.
Article in Ecotoxicology (London, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Selection and validation of suitable reference genes for qPCR expression analysis in youngFrontiers in plant science · 2026Article
- Phytoremediation potential of Brassica oleracea varieties through cadmium tolerance gene expression analysis.Journal, genetic engineering & biotechnology · 2024Article
- Genome-Wide Identification of the U-Box E3 Ubiquitin Ligase Gene Family in Cabbage (Plants (Basel, Switzerland) · 2023Article
- In-site and Ex-site Date Palm Exposure to Heavy Metals Involved Infra-Individual Biomarkers Upregulation.Plants (Basel, Switzerland) · 2021Article
- The role of epicuticular waxes on foliar metal transfer and phytotoxicity in edible vegetables: case of Brassica oleracea species exposed to manufactured particles.Environmental science and pollution research international · 2019Article
- Identification and Characterization of Three Epithiospecifier Protein Isoforms inFrontiers in plant science · 2019Article
- Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda).Scientific reports · 2018Article
- Selection of reference genes for reverse transcription-qPCR analysis in the biomonitor macrophytePhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Selection of reference genes suitable for normalization of qPCR data under abiotic stresses in bioenergy crop Arundo donax L.Scientific reports · 2017Article
- Effect of earthworms on growth, photosynthetic efficiency and metal uptake in Brassica juncea L. plants grown in cadmium-polluted soils.Environmental science and pollution research international · 2017Article
- Antioxidant defense gene analysis in Brassica oleracea and Trifolium repens exposed to Cd and/or Pb.Environmental science and pollution research international · 2016Article
- Evaluation and selection of reference genes for ecotoxicogenomic study of the green alga Closterium ehrenbergii using quantitative real-time PCR.Ecotoxicology (London, England) · 2015Article
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Abstract
Real-time quantitative PCR is nowadays a standard method to study gene expression variations in various samples and experimental conditions. However, to interpret results accurately, data normalization with appropriate reference genes appears to be crucial. The present study describes the identification and the validation of suitable reference genes in Brassica oleracea leaves. Expression stability of eight candidates was tested following drought and cold abiotic stresses by using three different softwares (BestKeeper, NormFinder and geNorm). Four genes (BolC.TUB6, BolC.SAND1, BolC.UBQ2 and BolC.TBP1) emerged as the most stable across the tested conditions. Further gene expression analysis of a drought- and a cold-responsive gene (BolC.DREB2A and BolC.ELIP, respectively), confirmed the stability and the reliability of the identified reference genes when used for normalization in the leaves of B. oleracea. These four genes were finally tested upon a benzene exposure and all appeared to be useful reference genes along this toxicological condition. These results provide a good starting point for future studies involving gene expression measurement on leaves of B. oleracea exposed to environmental modifications.
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