ArticleDevelopmental biology2014
Loss of Hand2 in a population of Periostin lineage cells results in pronounced bradycardia and neonatal death.
Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Rare TruncatingGenes · 2026Article
- Hand1 gene replacement with Hand2 reveals overlap in function with unique occurrence of omphalocele and heart defects.Development (Cambridge, England) · 2025Article
- Role of Maternal Obesity in Offspring Cardiovascular Development and Congenital Heart Defects.Journal of the American Heart Association · 2025Article
- The molecular mechanisms of cardiac development and related diseases.Signal transduction and targeted therapy · 2024Review
- Single cell evaluation of endocardial Hand2 gene regulatory networks reveals HAND2-dependent pathways that impact cardiac morphogenesis.Development (Cambridge, England) · 2023Article
- The adult heart requires baseline expression of the transcription factor Hand2 to withstand right ventricular pressure overload.Cardiovascular research · 2022Article
- HAND transcription factors cooperatively specify the aorta and pulmonary trunk.Developmental biology · 2021Article
- Periostin-expressing Schwann cells and endoneurial cardiac fibroblasts contribute to sympathetic nerve fasciculation after birth.Journal of molecular and cellular cardiology · 2021Article
- The diversity of neuronal phenotypes in rodent and human autonomic ganglia.Cell and tissue research · 2020Review
- Hand Factors in Cardiac Development.Anatomical record (Hoboken, N.J. : 2007) · 2019Review
- Striking parallels between carotid body glomus cell and adrenal chromaffin cell development.Developmental biology · 2018Article
- Limb-Enhancer Genie: An accessible resource of accurate enhancer predictions in the developing limb.PLoS computational biology · 2017Article
- HAND2 Target Gene Regulatory Networks Control Atrioventricular Canal and Cardiac Valve Development.Cell reports · 2017Article
- Genetic tools for identifying and manipulating fibroblasts in the mouse.Differentiation; research in biological diversity · 2016Review
- Molecular networks underlying myofibroblast fate and fibrosis.Journal of molecular and cellular cardiology · 2016Review
- Decellularized Human Skeletal Muscle as Biologic Scaffold for Reconstructive Surgery.International journal of molecular sciences · 2015Article
- Hand2 is an essential regulator for two Notch-dependent functions within the embryonic endocardium.Cell reports · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The Periostin Cre (Postn-Cre) lineage includes endocardial and neural crest derived mesenchymal cells of the cardiac cushions, neural crest-derived components of the sympathetic and enteric nervous systems, and cardiac fibroblasts. In this study, we use the Postn-Cre transgenic allele to conditionally ablate Hand2 (H2CKO). We find that Postn-Cre H2CKOs die shortly after birth despite a lack of obvious cardiac structural defects. To ascertain the cause of death, we performed a detailed comparison of the Postn-Cre lineage and Hand2 expression at mid and late stages of embryonic development. Gene expression analyses demonstrate that Postn-Cre ablates Hand2 from the adrenal medulla as well as the sphenopalatine ganglia of the head. In both cases, Hand2 loss-of-function dramatically reduces expression of Dopamine Beta Hydroxylase (Dbh), a gene encoding a crucial catecholaminergic biosynthetic enzyme. Expression of the genes Tyrosine Hydroxylase (Th) and Phenylethanolamine N-methyltransferase (Pnmt), which also encode essential catecholaminergic enzymes, were severely reduced in postnatal adrenal glands. Electrocardiograms demonstrate that 3-day postnatal Postn-Cre H2CKO pups exhibit sinus bradycardia. In conjunction with the aforementioned gene expression analyses, these results strongly suggest that the observed postnatal lethality occurs due to a catecholamine deficiency and subsequent heart failure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.