ArticleActa orthopaedica2014
Efficacy of a novel PCR- and microarray-based method in diagnosis of a prosthetic joint infection.
Article in Acta orthopaedica, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 46 citations in OpenAlex.
- Multiplex PCR test as an intra-operative diagnostic tool for periprosthetic joint infection in presumed aseptic revision hip and knee arthroplasty: a 1-year follow-up study of 200 cases.Journal of bone and joint infection · 2024Article
- Modulatory role of vitamins A, B3, C, D, and E on skin health, immunity, microbiome, and diseases.Pharmacological reports : PR · 2023Review
- Rapid Diagnostics of Joint Infections Using IS-Pro.Journal of clinical microbiology · 2023Article
- Usefulness of a Multiplex PCR Assay for the Diagnosis of Prosthetic Joint Infections in the Routine Setting.Orthopaedic surgery · 2022Article
- Challenges of antibiotic resistance biofilms and potential combating strategies: a review.3 Biotech · 2021Review
- An update about molecular biology techniques to detect orthopaedic implant-related infections.EFORT open reviews · 2021Review
- Are There Benefits In Early Diagnosis Of Prosthetic Joint Infection With Multiplex Polymerase Chain Reaction?Journal of bone and joint infection · 2017Article
- Editorial.Acta orthopaedica · 2016Article
- Microarray identification of bacterial species in peritonsillar abscesses.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- [Revision arthroplasty : Histopathological diagnostics in periprosthetic joint infections].Der Orthopade · 2015Article
- Molecular diagnostics.The Journal of the American Academy of Orthopaedic Surgeons · 2015Article
- Do crosslinking and vitamin E stabilization influence microbial adhesions on UHMWPE-based biomaterials?Clinical orthopaedics and related research · 2015Article
- Solid and Suspension Microarrays for Microbial Diagnostics.Methods in microbiology · 2015Article
- Diagnostic value of a PCR-based technique for prosthetic joint infection.Journal of clinical microbiology · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposePolymerase chain reaction (PCR) methods enable detection and species identification of many pathogens. We assessed the efficacy of a new PCR and microarray-based platform for detection of bacteria in prosthetic joint infections (PJIs).
methodsThis prospective study involved 61 suspected PJIs in hip and knee prostheses and 20 negative controls. 142 samples were analyzed by Prove-it Bone and Joint assay. The laboratory staff conducting the Prove-it analysis were not aware of the results of microbiological culture and clinical findings. The results of the analysis were compared with diagnosis of PJIs defined according to the Musculoskeletal Infection Society (MSIS) criteria and with the results of microbiological culture.
results38 of 61 suspected PJIs met the definition of PJI according to the MSIS criteria. Of the 38 patients, the PCR detected bacteria in 31 whereas bacterial culture was positive in 28 patients. 15 of the PJI patients were undergoing antimicrobial treatment as the samples for analysis were obtained. When antimicrobial treatment had lasted 4 days or more, PCR detected bacteria in 6 of the 9 patients, but positive cultures were noted in only 2 of the 9 patients. All PCR results for the controls were negative. Of the 61 suspected PJIs, there were false-positive PCR results in 6 cases.
interpretationThe Prove-it assay was helpful in PJI diagnostics during ongoing antimicrobial treatment. Without preceding treatment with antimicrobials, PCR and microarray-based assay did not appear to give any additional information over culture.
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