ArticleBiosensors2013
Sensing of EGTA Mediated Barrier Tissue Disruption with an Organic Transistor.
Article in Biosensors, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- TEER and Ion Selective Transwell-Integrated Sensors System for Caco-2 Cell Model.Micromachines · 2023Article
- Epithelium dynamics differ in time and space when exposed to the permeation enhancers penetramax and EGTA. A head-to-head mechanistic comparison.Frontiers in drug delivery · 2023Article
- Organic electronic transmembrane device for hosting and monitoring 3D cell cultures.Science advances · 2022Article
- Current-Driven Organic Electrochemical Transistors for Monitoring Cell Layer Integrity with Enhanced Sensitivity.Advanced healthcare materials · 2021Review
- Rapid Evaluation of Intestinal Paracellular Permeability Using the Human Enterocytic-Like Caco-2/TC7 Cell Line.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Substrate curvature induces fallopian tube epithelial cell invasion via cell-cell tension in a model of ovarian cortical inclusion cysts.Integrative biology : quantitative biosciences from nano to macro · 2019Article
- Transistor in a tube: A route to three-dimensional bioelectronics.Science advances · 2018Article
- DIE-RNA: A Reproducible Strategy for the Digestion of Normal and Injured Pancreas, Isolation of Pancreatic Cells from Genetically Engineered Mouse Models and Extraction of High Quality RNA.Frontiers in physiology · 2018Article
- Combined optical and electronic sensing of epithelial cells using planar organic transistors.Advanced materials (Deerfield Beach, Fla.) · 2014Article
- Sensing of barrier tissue disruption with an organic electrochemical transistor.Journal of visualized experiments : JoVE · 2014Article
- Special issue on organic electronic bio-devices.Biosensors · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Barrier tissue protects the body against external factors by restricting the passage of molecules. The gastrointestinal epithelium is an example of barrier tissue with the primary purpose of allowing the passage of ions and nutrients, while restricting the passage of pathogens and toxins. It is well known that the loss of barrier function can be instigated by a decrease in extracellular calcium levels, leading to changes in protein conformation and an increase in paracellular transport. In this study, ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetra acetic acid (EGTA), a calcium chelator, was used to disrupt the gastrointestinal epithelial barrier. The effect of EGTA on barrier tissue was monitored by a novel label-free method based on an organic electrochemical transistor (OECT) integrated with living cells and validated against conventional methods for measuring barrier tissue integrity. We demonstrate that the OECT can detect breaches in barrier tissue upon exposure to EGTA with the same sensitivity as existing methods but with increased temporal resolution. Due to the potential of low cost processing techniques and the flexibility in design associated with organic electronics, the OECT has great potential for high-throughput, disposable sensing and diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.