ArticleMolecular biotechnology2014
Real-time PCR quantification of the plant growth promoting bacteria Herbaspirillum seropedicae strain SmR1 in maize roots.
Article in Molecular biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Optimization of Molecular Methods for Detecting Duckweed-Associated Bacteria.Plants (Basel, Switzerland) · 2023Article
- Multiomic Approaches Reveal Hormonal Modulation and Nitrogen Uptake and Assimilation in the Initial Growth of Maize Inoculated withPlants (Basel, Switzerland) · 2022Article
- Inoculation of Herbaspirillum seropedicae strain SmR1 increases biomass in maize roots DKB 390 variety in the early stages of plant development.Archives of microbiology · 2022Article
- Sorghum Growth Promotion byMicroorganisms · 2020Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- The Endophytic Bacterial Microbiota Associated with Sweet Sorghum (International journal of genomics · 2018Article
- Effect ofPeerJ · 2018Article
- Article
- Differential growth responses of Brachypodium distachyon genotypes to inoculation with plant growth promoting rhizobacteria.Plant molecular biology · 2016Article
- Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR.Applied and environmental microbiology · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant growth promoting bacteria Herbaspirillum seropedicae SmR1 is an endophytic diazotroph found in several economically important crops. Considering that methods to monitor the plant-bacteria interaction are required, our objective was to develop a real-time PCR method for quantification of PGPB H. seropedicae in the rhizosphere of maize seedlings. Primer pairs were designed, and their specificity was verified using DNA from 12 different bacterial species. Ten standard curves of qPCR assay using HERBAS1 primers and tenfold serial dilutions of H. seropedicae SmR1 DNA were performed, and PCR efficiency of 91 % and correlation coefficient of 0.99 were obtained. H. seropedicae SmR1 limit of detection was 10(1) copies (corresponding to 60.3 fg of bacterial DNA). qPCR assay using HERBAS1 was used to detect and quantify H. seropedicae strain SmR1 in inoculated maize roots, cultivated in vitro and in pots, harvested 1, 4, 7, and 10 days after inoculation. The estimated bacterial DNA copy number per gram of root was in the range 10(7)-10(9) for plants grown in vitro and it was around 10(6) for plants grown in pots. Primer pair HERBAS1 was able to quantify H. seropedicae SmR1, and this assay can be useful for monitoring plant-bacteria interaction.
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