ArticleEukaryotic cell2014
Multiple nuclear localization signals mediate nuclear localization of the GATA transcription factor AreA.
Article in Eukaryotic cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 49 citations in OpenAlex.
- Engineering of Global Transcriptional Regulators (GTRs) inJournal of fungi (Basel, Switzerland) · 2025Review
- Article
- Multiple types of nuclear localization signals inBiochemistry and biophysics reports · 2024Article
- Regulation of nitrogen utilization and mycotoxin biosynthesis by the GATA transcription factor AaAreA in Alternaria alternata.World journal of microbiology & biotechnology · 2024Article
- Regulation of nutrient utilization in filamentous fungi.Applied microbiology and biotechnology · 2023Review
- A Gene fromJournal of fungi (Basel, Switzerland) · 2023Article
- Characterization of the nuclear import of the human CHD4-NuRD complex.Journal of cell science · 2023Article
- GATA-type transcriptional factor SpGAT1 interacts with SpMIG1 and promotes lipid accumulation in the oleaginous yeastBiotechnology for biofuels and bioproducts · 2022Article
- Co-option of an extracellular protease for transcriptional control of nutrient degradation in the fungus Aspergillus nidulans.Communications biology · 2021Article
- Characterization of the promoter of the nitrate transporter-encoding gene nrtA in Aspergillus nidulans.Molecular genetics and genomics : MGG · 2020Article
- Sequence analysis and protein interactions of Arabidopsis CIA2 and CIL proteins.Botanical studies · 2020Article
- A nuclear localization signal is required for the nuclear translocation of Fign and its microtubule‑severing function.Molecular medicine reports · 2020Article
- Distribution, evolution and expression ofPeerJ · 2020Article
- Article
- The role of the GATA transcription factor AreB in regulation of nitrogen and carbon metabolism in Aspergillus nidulans.FEMS microbiology letters · 2019Article
- More than One Way in: Three Gln3 Sequences Required To Relieve Negative Ure2 Regulation and Support Nuclear Gln3 Import inGenetics · 2018Article
- Identification of Plasmodesmal Localization Sequences in Proteins In Planta.Journal of visualized experiments : JoVE · 2017Article
- Distinctive Nuclear Localization Signals in the OomyceteFrontiers in microbiology · 2017Article
- The AreA transcription factor mediates the regulation of deoxynivalenol (DON) synthesis by ammonium and cyclic adenosine monophosphate (cAMP) signalling in Fusarium graminearum.Molecular plant pathology · 2015Article
- Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals.eLife · 2015Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
The Aspergillus nidulans GATA transcription factor AreA activates transcription of nitrogen metabolic genes in response to nitrogen limitation and is known to accumulate in the nucleus during nitrogen starvation. Sequence analysis of AreA revealed multiple nuclear localization signals (NLSs), five putative classical NLSs conserved in fungal AreA orthologs but not in the Saccharomyces cerevisiae functional orthologs Gln3p and Gat1p, and one putative noncanonical RRX33RXR bipartite NLS within the DNA-binding domain. In order to identify the functional NLSs in AreA, we constructed areA mutants with mutations in individual putative NLSs or combinations of putative NLSs and strains expressing green fluorescent protein (GFP)-AreA NLS fusion genes. Deletion of all five classical NLSs individually or collectively did not affect utilization of nitrogen sources or AreA-dependent gene expression and did not prevent AreA nuclear localization. Mutation of the bipartite NLS conferred the inability to utilize alternative nitrogen sources and abolished AreA-dependent gene expression likely due to effects on DNA binding but did not prevent AreA nuclear localization. Mutation of all six NLSs simultaneously prevented AreA nuclear accumulation. The bipartite NLS alone strongly directed GFP to the nucleus, whereas the classical NLSs collaborated to direct GFP to the nucleus. Therefore, AreA contains multiple conserved NLSs, which show redundancy and together function to mediate nuclear import. The noncanonical bipartite NLS is conserved in GATA factors from Aspergillus, yeast, and mammals, indicating an ancient origin.
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