ArticleEukaryotic cell2014
The Entamoeba histolytica Dnmt2 homolog (Ehmeth) confers resistance to nitrosative stress.
Article in Eukaryotic cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Microbiota-derived indole differentially shapes Entamoeba histolytica physiology and promotes host-compatible colonization.PLoS neglected tropical diseases · 2026Article
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- Molecular Coping Mechanisms: Reprogramming tRNAs To Regulate Codon-Biased Translation of Stress Response Proteins.Accounts of chemical research · 2023Article
- RNA methylation and cellular response to oxidative stress-promoting anticancer agents.Cell cycle (Georgetown, Tex.) · 2023Review
- Gene expression of axenically-isolated clinical Entamoeba histolytica strains and its impact on disease severity of amebiasis.PLoS pathogens · 2022Article
- Queuine Salvaging in the Human ParasiteCells · 2022Article
- Are Metabolites From the Gut Microbiota Capable of Regulating Epigenetic Mechanisms in the Human ParasiteFrontiers in cell and developmental biology · 2022Review
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- Phosphorylation of eukaryotic initiation factor-2α in response to endoplasmic reticulum and nitrosative stress in the human protozoan parasite, Entamoeba histolytica.Molecular and biochemical parasitology · 2019Article
- Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression.Redox biology · 2018Article
- Utilization of Different Omic Approaches to Unravel Stress Response Mechanisms in the ParasiteFrontiers in cellular and infection microbiology · 2018Review
- Mechanism and biological role of Dnmt2 in Nucleic Acid Methylation.RNA biology · 2017Review
- Identification of S-Nitrosylated (SNO) Proteins inFrontiers in cellular and infection microbiology · 2017Article
- Characterization of Cytosine Methylation and the DNA Methyltransferases ofInternational journal of biological sciences · 2017Article
- Phosphorylation of Eukaryotic Initiation Factor-2α during Stress and Encystation in Entamoeba Species.PLoS pathogens · 2016Article
- N-acetyl ornithine deacetylase is a moonlighting protein and is involved in the adaptation of Entamoeba histolytica to nitrosative stress.Scientific reports · 2016Article
- Deciphering the epitranscriptome: A green perspective.Journal of integrative plant biology · 2016Review
- Reviving the RNA World: An Insight into the Appearance of RNA Methyltransferases.Frontiers in genetics · 2016Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitric oxide (NO) has antimicrobial properties against many pathogens due to its reactivity as an S-nitrosylating agent. It inhibits many of the key enzymes that are involved in the metabolism and virulence of the parasite Entamoeba histolytica through S-nitrosylation of essential cysteine residues. Very little information is available on the mechanism of resistance to NO by pathogens in general and by this parasite in particular. Here, we report that exposure of the parasites to S-nitrosoglutathione (GSNO), an NO donor molecule, strongly reduces their viability and protein synthesis. However, the deleterious effects of NO were significantly reduced in trophozoites overexpressing Ehmeth, the cytosine-5 methyltransferase of the Dnmt2 family. Since these trophozoites also exhibited high levels of tRNA(Asp) methylation, the high levels suggested that Ehmeth-mediated tRNA(Asp) methylation is part of the resistance mechanism to NO. We previously reported that enolase, another glycolytic enzyme, binds to Ehmeth and inhibits its activity. We observed that the amount of Ehmeth-enolase complex was significantly reduced in GSNO-treated E. histolytica, which explains the aforementioned increase of tRNA methylation. Specifically, we demonstrated via site-directed mutagenesis that cysteine residues 228 and 229 of Ehmeth are susceptible to S-nitrosylation and are crucial for Ehmeth binding to enolase and for Ehmeth-mediated resistance to NO. These results indicate that Ehmeth has a central role in the response of the parasite to NO, and they contribute to the growing evidence that NO is a regulator of epigenetic mechanisms.
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