ArticleHistochemistry and cell biology2014
ROCK inhibition prevents early mouse embryo development.
Article in Histochemistry and cell biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- BACH1 recruits STAT3 to enhance leukemia inhibitory factor receptor activity and augments the self-renewal capacity of mouse embryonic stem cells.Stem cell research & therapy · 2025Article
- Signaling pathway regulators in preimplantation embryos.Journal of molecular histology · 2024Review
- Transcriptional Profiling of Porcine Blastocysts Produced In Vitro in a Chemically Defined Culture Medium.Animals : an open access journal from MDPI · 2021Article
- LIMK1/2 are required for actin filament and cell junction assembly in porcine embryos developing in vitro.Asian-Australasian journal of animal sciences · 2020Article
- CHK2 is essential for spindle assembly and DNA repair during the first cleavage of mouse embryos.Aging · 2020Article
- Rocking the Boat: The Decisive Roles of Rho Kinases During Oocyte, Blastocyst, and Stem Cell Development.Frontiers in cell and developmental biology · 2020Review
- Podophyllotoxin Exposure Causes Spindle Defects and DNA Damage-Induced Apoptosis in Mouse Fertilized Oocytes and Early Embryos.Frontiers in cell and developmental biology · 2020Article
- Temporal patterns of gene regulation and upstream regulators contributing to major developmental transitions during Rhesus macaque preimplantation development.Molecular human reproduction · 2019Article
- Nrf2 inhibition affects cell cycle progression during early mouse embryo development.The Journal of reproduction and development · 2018Article
- Involvement of LIMK1/2 in actin assembly during mouse embryo development.Cell cycle (Georgetown, Tex.) · 2018Article
- Inhibition of Rho‑associated protein kinase increases the ratio of formation of blastocysts from single human blastomeres.Molecular medicine reports · 2016Article
- Article
- The Histochemistry and Cell Biology pandect: the year 2014 in review.Histochemistry and cell biology · 2015Review
- Inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of Hippo signaling in the mouse blastocyst.Developmental biology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ROCK is a Rho-GTPase effector that is important for actin assembly and is involved in various cellular functions, including cell contraction, migration, motility, and tumor cell invasion. In this study, we investigated ROCK expression and function during early mouse embryo development. Inhibiting ROCK by Y-27632 treatment at the zygote stage resulted in first cleavage failure, and most embryos failed to develop to the 8-cell stage. When adding Y-27632 at the 8-cell stage, embryos failed to undergo compaction and could not develop into blastocysts. In addition, fluorescence staining intensity analysis indicated that actin expression at blastomere membranes was significantly reduced. After ROCK inhibition, two or more nuclei were observed in a cell, which indicated possible cytokinesis failure. Moreover, after ROCK inhibition with Y-27632, the phosphorylation levels of LIMK1/2, a downstream molecule of ROCK, were decreased at blastomere membranes. Thus, our results showed conserved roles for ROCK in this mammalian embryo model and indicated that a ROCK-LIMK1/2-actin pathway might regulate cleavage and blastocyst formation during early mouse embryo development.
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