ArticleNature methods2014
Gold rotor bead tracking for high-speed measurements of DNA twist, torque and extension.
Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Geometry of braided DNA dictates supercoiling partitioning.Nature communications · 2026Article
- Stepwise DNA-unwinding gates TnpB genome-editing activity.Molecular cell · 2026Article
- Protein force spectroscopy using magnetic tweezers: Slow and steady wins the race?Biophysical journal · 2026Review
- Stepwise DNA unwinding gates TnpB genome-editing activity.bioRxiv : the preprint server for biology · 2026Article
- Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing.Molecular cell · 2025Article
- Dynamic basis of supercoiling-dependent DNA interrogation by Cas12a via R-loop intermediates.Nature communications · 2025Article
- Tunable elliptical cylinders for rotational mechanical studies of single DNA molecules.Science advances · 2024Article
- Nucleosome Dynamics Derived at the Single-Molecule Level Bridges Its Structures and Functions.JACS Au · 2024Review
- Exploration of the Character Representation of DNA Chiral Conformations and Deformations via a Curved Surface Discrete Frenet Frame.International journal of molecular sciences · 2023Article
- The energy landscape for R-loop formation by the CRISPR-Cas Cascade complex.Nature structural & molecular biology · 2023Article
- DNA Origami: Recent Progress and Applications.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Microparticle Manipulation Based on the Bulk Acoustic Wave Combined with the Liquid Crystal Backflow Effect Driving in 2D/3D Platforms.ACS omega · 2022Article
- Optical control of fast and processive engineered myosins in vitro and in living cells.Nature chemical biology · 2021Article
- Optical tweezers in single-molecule biophysics.Nature reviews. Methods primers · 2021Article
- Estimation of rolling work of adhesion at the nanoscale with soft probing using optical tweezers.RSC advances · 2021Article
- Acoustic Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2020Review
- A benchmark data set for the mechanical properties of double-stranded DNA and RNA under torsional constraint.Data in brief · 2020Article
- Structure-mechanics statistical learning unravels the linkage between local rigidity and global flexibility in nucleic acids.Chemical science · 2020Article
- Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Insights into Kinesin-1 Stepping from Simulations and Tracking of Gold Nanoparticle-Labeled Motors.Biophysical journal · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Single-molecule measurements of DNA twist and extension have been used to reveal physical properties of the double helix and to characterize structural dynamics and mechanochemistry in nucleoprotein complexes. However, the spatiotemporal resolution of twist measurements has been limited by the use of angular probes with high rotational drag, which prevents detection of short-lived intermediates or small angular steps. We introduce gold rotor bead tracking (AuRBT), which yields >100× improvement in time resolution over previous techniques. AuRBT employs gold nanoparticles as bright low-drag rotational and extensional probes, which are monitored by instrumentation that combines magnetic tweezers with objective-side evanescent darkfield microscopy. Our analysis of high-speed structural dynamics of DNA gyrase using AuRBT revealed an unanticipated transient intermediate. AuRBT also enables direct measurements of DNA torque with >50× shorter integration times than previous techniques; we demonstrated high-resolution torque spectroscopy by mapping the conformational landscape of a Z-forming DNA sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.