ArticleOptics letters2014
Correction of depth-dependent aberrations in 3D single-molecule localization and super-resolution microscopy.
Article in Optics letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.Nature communications · 2025Article
- Universal inverse modeling of point spread functions for SMLM localization and microscope characterization.Nature methods · 2024Article
- Universal inverse modelling of point spread functions for SMLM localization and microscope characterization.bioRxiv : the preprint server for biology · 2023Article
- Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adultFrontiers in neuroscience · 2022Article
- Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function.The journal of physical chemistry. B · 2021Article
- Recent progress on single-molecule localization microscopy.Biophysics reports · 2021Article
- Adaptive optics in super-resolution microscopy.Biophysics reports · 2021Article
- Addressing systematic errors in axial distance measurements in single-emitter localization microscopy.Optics express · 2020Article
- Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.Annual review of biomedical engineering · 2020Review
- Comparing strategies for deep astigmatism-based single-molecule localization microscopy.Biomedical optics express · 2020Article
- Feedforward Control for Single Particle Tracking Synthetic Motion.IFAC-PapersOnLine · 2020Article
- Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization.Biomedical optics express · 2019Article
- Combining 3D single molecule localization strategies for reproducible bioimaging.Nature communications · 2019Article
- Q&A: Array tomography.BMC biology · 2018Article
- Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections.Nature methods · 2018Article
- Imaging the three-dimensional orientation and rotational mobility of fluorescent emitters using the Tri-spot point spread function.Applied physics letters · 2018Article
- Self-interference 3D super-resolution microscopy for deep tissue investigations.Nature methods · 2018Article
- Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells [Invited].Optics express · 2018Review
- Super-Resolution Microscopy: Shedding Light on the Cellular Plasma Membrane.Chemical reviews · 2017Review
- Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.Chemical reviews · 2017Review
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4 authors.
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Abstract
Single-molecule switching based super-resolution microscopy techniques have been extended into three dimensions through various 3D single-molecule localization methods. However, the localization accuracy in z can be severely degraded by the presence of aberrations, particularly the spherical aberration introduced by the refractive index mismatch when imaging into an aqueous sample with an oil immersion objective. This aberration confines the imaging depth in most experiments to regions close to the coverslip. Here we show a method to obtain accurate, depth-dependent z calibrations by measuring the point spread function (PSF) at the coverslip surface, calculating the microscope pupil function through phase retrieval, and then computing the depth-dependent PSF with the addition of spherical aberrations. We demonstrate experimentally that this method can maintain z localization accuracy over a large range of imaging depths. Our super-resolution images of a mammalian cell nucleus acquired between 0 and 2.5 μm past the coverslip show that this method produces accurate z localizations even in the deepest focal plane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.