ArticleJournal of visualized experiments : JoVE2014
Using microfluidics chips for live imaging and study of injury responses in Drosophila larvae.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Immobilizing Second-InstarCold Spring Harbor protocols · 2025Article
- Patronin/CAMSAP promotes reactivation and regeneration of Drosophila quiescent neural stem cells.EMBO reports · 2023Article
- Focal laser stimulation of fly nociceptors activates distinct axonal and dendritic CaBiophysical journal · 2021Article
- Modeling Neurodegenerative Disorders inInternational journal of molecular sciences · 2020Review
- Long-term in vivo imaging of Drosophila larvae.Nature protocols · 2020Article
- LarvaSPA, A Method for Mounting Drosophila Larva for Long-Term Time-Lapse Imaging.Journal of visualized experiments : JoVE · 2020Article
- Article
- Collective behaviors of Drosophila-derived retinal progenitors in controlled microenvironments.PloS one · 2019Article
- A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2018Article
- Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans.Journal of visualized experiments : JoVE · 2018Article
- A Simple Neuronal Mechanical Injury Methodology to Study Drosophila Motor Neuron Degeneration.Journal of visualized experiments : JoVE · 2017Article
- On chip cryo-anesthesia of Drosophila larvae for high resolution in vivo imaging applications.Lab on a chip · 2017Article
- Methodological advances in imaging intravital axonal transport.F1000Research · 2017Review
- Agar-polydimethylsiloxane devices for quantitative investigation of oviposition behaviour of adult Drosophila melanogaster.Biomicrofluidics · 2015Article
- Drosophila Neuronal Injury Follows a Temporal Sequence of Cellular Events Leading to Degeneration at the Neuromuscular Junction.Journal of experimental neuroscience · 2015Article
- Article
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Authors and funding
8 authors.
Funding
Abstract
Live imaging is an important technique for studying cell biological processes, however this can be challenging in live animals. The translucent cuticle of the Drosophila larva makes it an attractive model organism for live imaging studies. However, an important challenge for live imaging techniques is to noninvasively immobilize and position an animal on the microscope. This protocol presents a simple and easy to use method for immobilizing and imaging Drosophila larvae on a polydimethylsiloxane (PDMS) microfluidic device, which we call the 'larva chip'. The larva chip is comprised of a snug-fitting PDMS microchamber that is attached to a thin glass coverslip, which, upon application of a vacuum via a syringe, immobilizes the animal and brings ventral structures such as the nerve cord, segmental nerves, and body wall muscles, within close proximity to the coverslip. This allows for high-resolution imaging, and importantly, avoids the use of anesthetics and chemicals, which facilitates the study of a broad range of physiological processes. Since larvae recover easily from the immobilization, they can be readily subjected to multiple imaging sessions. This allows for longitudinal studies over time courses ranging from hours to days. This protocol describes step-by-step how to prepare the chip and how to utilize the chip for live imaging of neuronal events in 3(rd) instar larvae. These events include the rapid transport of organelles in axons, calcium responses to injury, and time-lapse studies of the trafficking of photo-convertible proteins over long distances and time scales. Another application of the chip is to study regenerative and degenerative responses to axonal injury, so the second part of this protocol describes a new and simple procedure for injuring axons within peripheral nerves by a segmental nerve crush.
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Registered trials
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