ArticleNucleic acids research2014
Molecular basis for the wide range of affinity found in Csr/Rsm protein-RNA recognition.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Functional analysis of natural variation in the RNA-binding protein CsrA across the bacterial domain predicts regulatory activity.Nucleic acids research · 2026Article
- Newton's cradle-like allosteric mechanism explains regulatory RsmE RNA binding.Nature communications · 2026Article
- Review
- The GacS/GacA two-component system strongly regulates antimicrobial competition mechanisms ofJournal of bacteriology · 2025Article
- TheFrontiers in cellular and infection microbiology · 2025Article
- Molecular Dynamics and Docking Simulations of Homologous RsmE Methyltransferases Hints at a General Mechanism for Substrate Release upon Uridine Methylation on 16S rRNA.International journal of molecular sciences · 2023Article
- Structural basis of sRNA RsmZ regulation of Pseudomonas aeruginosa virulence.Cell research · 2023Article
- Plasmids manipulate bacterial behaviour through translational regulatory crosstalk.PLoS biology · 2023Article
- Spatial Structure Formation by RsmE-Regulated Extracellular Secretions in Pseudomonas fluorescens Pf0-1.Journal of bacteriology · 2022Article
- Pre-mRNA splicing factor U2AF2 recognizes distinct conformations of nucleotide variants at the center of the pre-mRNA splice site signal.Nucleic acids research · 2022Article
- ProNAB: database for binding affinities of protein-nucleic acid complexes and their mutants.Nucleic acids research · 2022Article
- Prokaryotic ncRNAs: Master regulators of gene expression.Current research in pharmacology and drug discovery · 2022Article
- Global identification of RsmA/N binding sites inRNA biology · 2021Article
- Exploring the expression and functionality of theRNA biology · 2021Article
- Phloroglucinol Derivatives in Plant-BeneficialMetabolites · 2021Review
- Three Ribosomal Operons ofFrontiers in microbiology · 2021Article
- Genome-Wide Analysis of Targets for Post-Transcriptional Regulation by Rsm Proteins inFrontiers in molecular biosciences · 2021Article
- RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression.PLoS pathogens · 2020Article
- Bactericidal type IV secretion system homeostasis in Xanthomonas citri.PLoS pathogens · 2020Article
- RNA-Binding Proteins Driving the Regulatory Activity of Small Non-coding RNAs in Bacteria.Frontiers in molecular biosciences · 2020Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The carbon storage regulator/regulator of secondary metabolism (Csr/Rsm) type of small non-coding RNAs (sRNAs) is widespread throughout bacteria and acts by sequestering the global translation repressor protein CsrA/RsmE from the ribosome binding site of a subset of mRNAs. Although we have previously described the molecular basis of a high affinity RNA target bound to RsmE, it remains unknown how other lower affinity targets are recognized by the same protein. Here, we have determined the nuclear magnetic resonance solution structures of five separate GGA binding motifs of the sRNA RsmZ of Pseudomonas fluorescens in complex with RsmE. The structures explain how the variation of sequence and structural context of the GGA binding motifs modulate the binding affinity for RsmE by five orders of magnitude (∼10 nM to ∼3 mM, Kd). Furthermore, we see that conformational adaptation of protein side-chains and RNA enable recognition of different RNA sequences by the same protein contributing to binding affinity without conferring specificity. Overall, our findings illustrate how the variability in the Csr/Rsm protein-RNA recognition allows a fine-tuning of the competition between mRNAs and sRNAs for the CsrA/RsmE protein.
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