ArticleJournal of visualized experiments : JoVE2014
Sorting of Streptomyces cell pellets using a complex object parametric analyzer and sorter.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
5 citing papers in PubMed.
- Current Understanding on Adhesion and Biofilm Development inInternational journal of microbiology · 2021Review
- Streptomyces Differentiation in Liquid Cultures as a Trigger of Secondary Metabolism.Antibiotics (Basel, Switzerland) · 2018Review
- SParticle, an algorithm for the analysis of filamentous microorganisms in submerged cultures.Antonie van Leeuwenhoek · 2018Article
- Aggregation of germlings is a major contributing factor towards mycelial heterogeneity of Streptomyces.Scientific reports · 2016Article
- The DyP-type peroxidase DtpA is a Tat-substrate required for GlxA maturation and morphogenesis in Streptomyces.Open biology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptomycetes are filamentous soil bacteria that are used in industry for the production of enzymes and antibiotics. When grown in bioreactors, these organisms form networks of interconnected hyphae, known as pellets, which are heterogeneous in size. Here we describe a method to analyze and sort mycelial pellets using a Complex Object Parametric Analyzer and Sorter (COPAS). Detailed instructions are given for the use of the instrument and the basic statistical analysis of the data. We furthermore describe how pellets can be sorted according to user-defined settings, which enables downstream processing such as the analysis of the RNA or protein content. Using this methodology the mechanism underlying heterogeneous growth can be tackled. This will be instrumental for improving streptomycetes as a cell factory, considering the fact that productivity correlates with pellet size.
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Registered trials
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