ArticleApplied and environmental microbiology2014
Dimethylglycine provides salt and temperature stress protection to Bacillus subtilis.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Limits of a Glycine Betaine-Derived Xenobiotic as a Trojan Horse Antimicrobial.International journal of molecular sciences · 2026Article
- Bacillus inoculation drives fine-scale taxonomic shifts in the bacterial communities of saline-alkaline wheat rhizosphere soil.Environmental microbiome · 2026Article
- Localization and Single Molecule Dynamics ofCells · 2025Article
- Enhanced catabolism of glycine betaine and derivatives provides improved osmotic stress protection inApplied and environmental microbiology · 2024Article
- Anatomical and Metabolome Features ofInternational journal of molecular sciences · 2024Article
- Transcriptome-wide probing reveals RNA thermometers that regulate translation of glycerol permease genes inRNA (New York, N.Y.) · 2023Article
- Integrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria.Nature communications · 2023Article
- Pan-metagenome reveals the abiotic stress resistome of cigar tobacco phyllosphere microbiome.Frontiers in plant science · 2023Article
- Orchestrated Response of Intracellular Zwitterionic Metabolites in Stress Adaptation of the Halophilic Heterotrophic BacteriumMarine drugs · 2022Article
- Transcriptome analysis of L-leucine-producing Corynebacterium glutamicum under the addition of trimethylglycine.Amino acids · 2022Article
- Genome mining reveals abiotic stress resistance genes in plant genomes acquired from microbesFrontiers in plant science · 2022Article
- Bacteria and Archaea Synergistically Convert Glycine Betaine to Biogenic Methane in the Formosa Cold Seep of the South China Sea.mSystems · 2021Article
- The organosulfur compound dimethylsulfoniopropionate (DMSP) is utilized as an osmoprotectant byApplied and environmental microbiology · 2021Article
- Stressed out: Bacterial response to high salinity using compatible solute biosynthesis and uptake systems, lessons fromComputational and structural biotechnology journal · 2021Review
- Investigations of Dimethylglycine, Glycine Betaine, and Ectoine Uptake by a Betaine-Carnitine-Choline Transporter Family Transporter with Diverse Substrate Specificity inJournal of bacteriology · 2020Article
- Role ofApplied and environmental microbiology · 2020Article
- Genomic Blueprint of Glycine Betaine Metabolism in Coral Metaorganisms and Their Contribution to Reef Nitrogen Budgets.iScience · 2020Article
- Whole Genome Sequencing and Comparative Genomic Analyses ofMicroorganisms · 2020Article
- Drought-induced soil microbial amino acid and polysaccharide change and their implications for C-N cycles in a climate change world.Scientific reports · 2019Article
- Biosynthesis and uptake of glycine betaine as cold-stress response to low temperature in fish pathogen Vibrio anguillarum.Journal of microbiology (Seoul, Korea) · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycine betaine is a potent osmotic and thermal stress protectant of many microorganisms. Its synthesis from glycine results in the formation of the intermediates monomethylglycine (sarcosine) and dimethylglycine (DMG), and these compounds are also produced when it is catabolized. Bacillus subtilis does not produce sarcosine or DMG, and it cannot metabolize these compounds. Here we have studied the potential of sarcosine and DMG to protect B. subtilis against osmotic, heat, and cold stress. Sarcosine, a compatible solute that possesses considerable protein-stabilizing properties, did not serve as a stress protectant of B. subtilis. DMG, on the other hand, proved to be only moderately effective as an osmotic stress protectant, but it exhibited good heat stress-relieving and excellent cold stress-relieving properties. DMG is imported into B. subtilis cells primarily under osmotic and temperature stress conditions via OpuA, a member of the ABC family of transporters. Ligand-binding studies with the extracellular solute receptor (OpuAC) of the OpuA system showed that OpuAC possesses a moderate affinity for DMG, with a Kd value of approximate 172 μM; its Kd for glycine betaine is about 26 μM. Docking studies using the crystal structures of the OpuAC protein with the sulfur analog of DMG, dimethylsulfonioacetate, as a template suggest a model of how the DMG molecule can be stably accommodated within the aromatic cage of the OpuAC ligand-binding pocket. Collectively, our data show that the ability to acquire DMG from exogenous sources under stressful environmental conditions helps the B. subtilis cell to cope with growth-restricting osmotic and temperature challenges.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.