ArticleJournal of visualized experiments : JoVE2014
Rapid and efficient zebrafish genotyping using PCR with high-resolution melt analysis.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Ectopic expression of Myomaker and Myomixer in slow muscle cells induces slow muscle fusion and myofiber death.Journal of genetics and genomics = Yi chuan xue bao · 2024Article
- A simple and scalable zebrafish model of Sonic hedgehog medulloblastoma.Cell reports · 2024Article
- Stat3 Regulates Developmental Hematopoiesis and Impacts Myeloid Cell Function via Canonical and Non-Canonical Modalities.Journal of innate immunity · 2024Article
- Article
- AP-4 loss in CRISPR-edited zebrafish affects early embryo development.Advances in biological regulation · 2023Article
- A Rapid and Noninvasive Method That Extracts Polymerase Chain Reaction-Ready Genomic DNA from Adult Zebrafish.Zebrafish · 2022Article
- Article
- Pathogenic Effect ofNeurology · 2021Article
- Genetic variant of TTLL11 gene and subsequent ciliary defects are associated with idiopathic scoliosis in a 5-generation UK family.Scientific reports · 2021Article
- Metabolic rerouting via SCD1 induction impacts X-linked adrenoleukodystrophy.The Journal of clinical investigation · 2021Article
- Tricaine Methanesulfonate (MS222) Has Short-Term Effects on Young Adult Zebrafish (Frontiers in behavioral neuroscience · 2021Article
- Vanishing white matter disease expression of truncated EIF2B5 activates induced stress response.eLife · 2020Article
- Skin swabbing is a refined technique to collect DNA from model fish species.Scientific reports · 2020Article
- Article
- An automated system for rapid cellular extraction from live zebrafish embryos and larvae: Development and application to genotyping.PloS one · 2018Article
- Article
- A zebrafish model of X-linked adrenoleukodystrophy recapitulates key disease features and demonstrates a developmental requirement for abcd1 in oligodendrocyte patterning and myelination.Human molecular genetics · 2017Article
- Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing.Journal of visualized experiments : JoVE · 2017Article
- A Low-Cost Method of Skin Swabbing for the Collection of DNA Samples from Small Laboratory Fish.Zebrafish · 2017Article
- A Serotonin Circuit Acts as an Environmental Sensor to Mediate Midline Axon Crossing through EphrinB2.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
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Authors and funding
5 authors.
Funding
Abstract
Zebrafish is a powerful vertebrate model system for studying development, modeling disease, and performing drug screening. Recently a variety of genetic tools have been introduced, including multiple strategies for inducing mutations and generating transgenic lines. However, large-scale screening is limited by traditional genotyping methods, which are time-consuming and labor-intensive. Here we describe a technique to analyze zebrafish genotypes by PCR combined with high-resolution melting analysis (HRMA). This approach is rapid, sensitive, and inexpensive, with lower risk of contamination artifacts. Genotyping by PCR with HRMA can be used for embryos or adult fish, including in high-throughput screening protocols.
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Registered trials
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