Evidence map›Paper›PMID 24561003›Full record

ArticleToxicology2014

Astrocytes protect against diazinon- and diazoxon-induced inhibition of neurite outgrowth by regulating neuronal glutathione.

Daniella M Pizzurro, Khoi Dao, Lucio G Costa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
10.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 41 citations in OpenAlex.

  1. Review
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  10. Molecular pharmacology · 2017
    Article
  11. Review
  12. Article
  13. Article
  14. The role of oxidative stress in organophosphate and nerve agent toxicity.Annals of the New York Academy of Sciences · 2016
    Review
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  16. Review
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 2 countries.

Daniella M PizzurroDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
Khoi DaoDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA.
Lucio G CostaDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, USA; Department of Neuroscience, University of Parma, Parma, Italy. Electronic address: lgcosta@u.washington.edu.
University of Washington · US

Funding

XENOBIOTIC BIOTRANSFORMATION AND DISPOSITIONP30ES007033 · NIEHS · UNIVERSITY OF WASHINGTON · PI ALI SHOJAIE · 1995 to 2026
$42.5M
Environmetal Pathology/Toxicology Training ProgramT32ES007032 · NIEHS · UNIVERSITY OF WASHINGTON · PI FAUSTMAN, ELAINE M, ROSENFELD, MICHAEL E · 1985 to 2023
$10.7M
STUDY OF CELLULAR &MOLECULAR ASPECTS OF PESTICIDE NEURODEVELOPMENTAL TOXICITYP01ES009601 · NIEHS · UNIVERSITY OF WASHINGTON · PI FAUSTMAN, ELAINE M · 1999 to 2013
$8.7M
NIEHS NIH HHS P01 ES009601NIEHS NIH HHS P01ES009601NIEHS NIH HHS P30 ES007033NIEHS NIH HHS T32 ES007032NIEHS NIH HHS T32ES007032-35
6 · The paper itself

Abstract

Evidence demonstrating that human exposure to various organophosphorus insecticides (OPs) is associated with neurobehavioral deficits in children continues to emerge. The present study focused on diazinon (DZ) and its active oxygen metabolite, diazoxon (DZO), and explored their ability to impair neurite outgrowth in rat primary hippocampal neurons as a mechanism of developmental neurotoxicity. Both DZ and DZO (0.5-10 μM) significantly inhibited neurite outgrowth in hippocampal neurons, at concentrations devoid of any cyototoxicity. These effects appeared to be mediated by oxidative stress, as they were prevented by antioxidants (melatonin, N-t-butyl-alpha-phenylnitrone, and glutathione ethyl ester). Inhibition of neurite outgrowth was observed at concentrations below those required to inhibit the catalytic activity of acetylcholinesterase. The presence of astrocytes in the culture was able to provide protection against inhibition of neurite outgrowth by DZ and DZO. Astrocytes increased neuronal glutathione (GSH) in neurons, to levels comparable to those of GSH ethyl ester. Astrocytes depleted of GSH by L-buthionine-(S,R)-sulfoximine no longer conferred protection against DZ- and DZO-induced inhibition of neurite outgrowth. The findings indicate that DZ and DZO inhibit neurite outgrowth in hippocampal neurons by mechanisms involving oxidative stress, and that these effects can be modulated by astrocytes and astrocyte-derived GSH. Oxidative stress from other chemical exposures, as well as genetic abnormalities that result in deficiencies in GSH synthesis and regulation, may render individuals more susceptible to these developmental neurotoxic effects of OPs.

Indexed as

AcetylcholinesteraseAnimalsAstrocytesCells, CulturedCell SurvivalDiazinonGlutathioneHippocampusInsecticidesNeuritesNeuronsOrganophosphorus CompoundsRatsAcetylcholinesteraseDiazinondiazoxonGlutathioneInsecticidesOrganophosphorus CompoundsS-ethyl glutathioneDiazinonDiazoxonGlial–neuronal interactionsGlutathioneNeurite outgrowthOrganophosphorus insecticide

Identifiers

PMID24561003
PMCPMC3999384
OpenAlexW1979431543

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.