ArticleBiophysical journal2014
Simultaneous Raman microspectroscopy and fluorescence imaging of bone mineralization in living zebrafish larvae.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- Raman-based label-free microscopic analysis of the pancreas in living zebrafish larvae.FEBS open bio · 2025Article
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- Recent Advances in Bioimage Analysis Methods for Detecting Skeletal Deformities in Biomedical and Aquaculture Fish Species.Biomolecules · 2023Review
- Label-free structural and functional volumetric imaging by dual-modality optical-Raman projection tomography.Science advances · 2023Article
- Cementing COPNAS nexus · 2023Article
- Biomineralogical signatures of breast microcalcifications.Science advances · 2023Article
- Zebrafish Models to Study Ectopic Calcification and Calcium-Associated Pathologies.International journal of molecular sciences · 2023Review
- Article
- High resolution 3D structures of mineralized tissues in health and disease.Nature reviews. Endocrinology · 2021Review
- Applications of Vibrational Spectroscopy for Analysis of Connective Tissues.Molecules (Basel, Switzerland) · 2021Review
- Influence of Prednisolone and Alendronate on theJBMR plus · 2021Article
- In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy.Nature communications · 2020Article
- Transformation of bone mineral morphology: From discrete marquise-shaped motifs to a continuous interwoven mesh.Bone reports · 2020Article
- Introduction to Infrared and Raman-Based Biomedical Molecular Imaging and Comparison with Other Modalities.Molecules (Basel, Switzerland) · 2020Review
- Surface-Enhanced Raman Scattering Microspectroscopy Enables the Direct Characterization of Biomineral-Associated Organic Material on Single Calcareous Microskeletons.The journal of physical chemistry letters · 2020Article
- Raman Microscopy: Progress in Research on Cancer Cell Sensing.Sensors (Basel, Switzerland) · 2020Review
- Perspective on optical imaging for functional assessment in musculoskeletal extremity trauma surgery.Journal of biomedical optics · 2020Article
- Fourier transform infrared spectroscopy of developing bone mineral: from amorphous precursor to mature crystal.The Analyst · 2020Article
- VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy.Optics express · 2019Article
- Bone quality assessment of osteogenic cell cultures by Raman microscopy.Journal of Raman spectroscopy : JRS · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Confocal Raman microspectroscopy and fluorescence imaging are two well-established methods providing functional insight into the extracellular matrix and into living cells and tissues, respectively, down to single molecule detection. In living tissues, however, cells and extracellular matrix coexist and interact. To acquire information on this cell-matrix interaction, we developed a technique for colocalized, correlative multispectral tissue analysis by implementing high-sensitivity, wide-field fluorescence imaging on a confocal Raman microscope. As a proof of principle, we study early stages of bone formation in the zebrafish (Danio rerio) larvae because the zebrafish has emerged as a model organism to study vertebrate development. The newly formed bones were stained using a calcium fluorescent marker and the maturation process was imaged and chemically characterized in vivo. Results obtained from early stages of mineral deposition in the zebrafish fin bone unequivocally show the presence of hydrogen phosphate containing mineral phases in addition to the carbonated apatite mineral. The approach developed here opens significant opportunities in molecular imaging of metabolic activities, intracellular sensing, and trafficking as well as in vivo exploration of cell-tissue interfaces under (patho-)physiological conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.