ArticleBMC genomics2014
Identification of novel and conserved miRNAs involved in pollen development in Brassica campestris ssp. chinensis by high-throughput sequencing and degradome analysis.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 73 citations in OpenAlex.
- bra-miR9569 Targets thePlants (Basel, Switzerland) · 2025Article
- '140R' Rootstock Regulates Resveratrol Content in 'Cabernet Sauvignon' Grapevine Leaves Through miRNA.Plants (Basel, Switzerland) · 2024Article
- Cytological Observation and RNA-Seq Analyses RevealPlants (Basel, Switzerland) · 2024Article
- Disruption of the Contents of Endogenous Hormones Cause Pollen Development Obstruction and Abortion in Male-Sterile Hybrid Lily Populations.Plants (Basel, Switzerland) · 2023Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- High-temperature stress in crops: male sterility, yield loss and potential remedy approaches.Plant biotechnology journal · 2023Review
- Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing.BMC plant biology · 2021Article
- Article
- Hydrogen Sulfide Alleviates Alkaline Salt Stress by Regulating the Expression of MicroRNAs inFrontiers in plant science · 2021Article
- Turnip mosaic virus in oilseed rape activates networks of sRNA-mediated interactions between viral and host genomes.Communications biology · 2020Article
- miRNAs involved in transcriptome remodeling during pollen development and heat stress response in Solanum lycopersicum.Scientific reports · 2020Article
- Identification and profiling of microRNAs and differentially expressed genes during anther development between a genetic male-sterile mutant and its wildtype cotton via high-throughput RNA sequencing.Molecular genetics and genomics : MGG · 2020Article
- Novel plant microRNAs from broccoletti sprouts do not show cross-kingdom regulation of pancreatic cancer.Oncotarget · 2020Article
- Article
- Investigation of the genes associated with a male sterility mutant (msm) in Chinese cabbage (Brassica campestris ssp. pekinensis) using RNA-Seq.Molecular genetics and genomics : MGG · 2020Article
- Article
- Evolution of MIR159/319 genes in Brassica campestris and their function in pollen development.Plant molecular biology · 2019Article
- Identification of conserved and novel miRNAs responsive to heat stress in flowering Chinese cabbage using high-throughput sequencing.Scientific reports · 2019Article
- CircRNA Expression Pattern and ceRNA and miRNA-mRNA Networks Involved in Anther Development in the CMS Line ofInternational journal of molecular sciences · 2019Article
- Differential expression of miRNAs and their targets in wax-deficient rapeseed.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundmicroRNAs (miRNAs) are endogenous, noncoding, small RNAs that have essential regulatory functions in plant growth, development, and stress response processes. However, limited information is available about their functions in sexual reproduction of flowering plants. Pollen development is an important process in the life cycle of a flowering plant and is a major factor that affects the yield and quality of crop seeds.
resultsThis study aims to identify miRNAs involved in pollen development. Two independent small RNA libraries were constructed from the flower buds of the male sterile line (Bcajh97-01A) and male fertile line (Bcajh97-01B) of Brassica campestris ssp. chinensis. The libraries were subjected to high-throughput sequencing by using the Illumina Solexa system. Eight novel miRNAs on the other arm of known pre-miRNAs, 54 new conserved miRNAs, and 8 novel miRNA members were identified. Twenty-five pairs of novel miRNA/miRNA* were found. Among all the identified miRNAs, 18 differentially expressed miRNAs with over two-fold change between flower buds of male sterile line (Bcajh97-01A) and male fertile line (Bcajh97-01B) were identified. qRT-PCR analysis revealed that most of the differentially expressed miRNAs were preferentially expressed in flower buds of the male fertile line (Bcajh97-01B). Degradome analysis showed that a total of 15 genes were predicted to be the targets of seven miRNAs.
conclusionsOur findings provide an overview of potential miRNAs involved in pollen development and interactions between miRNAs and their corresponding targets, which may provide important clues on the function of miRNAs in pollen development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.