ArticleEnvironmental science & technology2014
Transcriptional and epigenetic mechanisms underlying enhanced in vitro adipocyte differentiation by the brominated flame retardant BDE-47.
Article in Environmental science & technology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it, 133 citations in OpenAlex.
- Prenatal exposure to persistent organic pollutants and childhood obesity: A systematic review and meta-analysis of human studies.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2022Pooled it
- Obesogens in Prostate Cancer: An Endocrine and Metabolic Threat.Current obesity reports · 2026Review
- Semi-automated, evidence-based workflow for selection of reference chemicals for the validation of NAMs: a case study with the adipogenesis assay.NAM journal · 2026Article
- Dietary Exposure to 2,2',4,4'-Tetrabromodiphenyl Ether (BDE-47) Causes Inflammation in the Liver of Common Carp (International journal of molecular sciences · 2025Article
- Endocrine Disruptors in Child Obesity and Related Disorders: Early Critical Windows of Exposure.Current nutrition reports · 2025Review
- Article
- Defining the role of 2,2',4,4'-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis.Adipocyte · 2024Article
- Polybrominated diphenyl ethers and gestational weight gain: a multi-center prospective cohort study.BJOG : an international journal of obstetrics and gynaecology · 2024Article
- Maternal Exposure to Low-Dose BDE-47 Induced Weight Gain and Impaired Insulin Sensitivity in the Offspring.International journal of molecular sciences · 2024Article
- Environmental Obesogens and Their Perturbations in Lipid Metabolism.Environment & health (Washington, D.C.) · 2024Review
- Toxic Effects and Mechanisms of Polybrominated Diphenyl Ethers.International journal of molecular sciences · 2023Review
- Prenatal airborne polycyclic aromatic hydrocarbon exposure, altered regulation of peroxisome proliferator-activated receptor gamma (Ppar)γ, and links with mammary cancer.Environmental research · 2023Article
- Polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, pesticides, and diabetes in the Anniston Community Health Survey follow-up (ACHS II): single exposure and mixture analysis approaches.The Science of the total environment · 2023Article
- Development of new approach methods for the identification and characterization of endocrine metabolic disruptors-a PARC project.Frontiers in toxicology · 2023Article
- Article
- Prenatal exposure to polybrominated diphenyl ethers and BMI Z-scores from 5 to 14 years.Environmental health : a global access science source · 2022Article
- Obesity II: Establishing causal links between chemical exposures and obesity.Biochemical pharmacology · 2022Review
- The Role of Persistent Organic Pollutants in Obesity: A Review of Laboratory and Epidemiological Studies.Toxics · 2022Review
- Developmental exposure to indoor flame retardants and hypothalamic molecular signatures: Sex-dependent reprogramming of lipid homeostasis.Frontiers in endocrinology · 2022Article
- Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 4 countries.
Funding
Abstract
Recent studies suggest that exposure to endocrine-disrupting compounds (EDCs) may play a role in the development of obesity. EDCs such as the flame retardant 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) have been shown to enhance adipocyte differentiation in the murine 3T3-L1 model. The mechanisms by which EDCs direct preadipocytes to form adipocytes are poorly understood. Here, we examined transcriptional and epigenetic mechanisms underlying the induction of in vitro adipocyte differentiation by BDE-47. Quantitative high content microscopy revealed concentration-dependent enhanced adipocyte differentiation following exposure to BDE-47 or the antidiabetic drug troglitazone (TROG). BDE-47 modestly activated the key adipogenic transcription factor peroxisome proliferator-activated receptor gamma (PPARγ) in COS7 cells, transiently transfected with a GAL4 reporter construct. Increased gene expression was observed for Pparγ2, leptin (Lep), and glucose-6-phophatase catalytic subunit (G6pc) in differentiated 3T3-L1 cells after BDE-47 exposure compared to TROG. Methylation-sensitive high resolution melting (MS-HRM) revealed significant demethylation of three CpG sites in the Pparγ2 promoter after exposure to both BDE-47 and TROG in differentiated 3T3-L1 cells. This study shows the potential of BDE-47 to induce adipocyte differentiation through various mechanisms that include Pparγ2 gene induction and promoter demethylation accompanied by activation of PPARγ, and possible disruption of glucose homeostasis and IGF1 signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.