ArticleParasites & vectors2014
First molecular characterization of Cryptosporidium and Giardia from bovines (Bos taurus and Bubalus bubalis) in Sri Lanka: unexpected absence of C. parvum from pre-weaned calves.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals.PLoS neglected tropical diseases · 2025Pooled it
- Prevalence and molecular identification ofOpen veterinary journal · 2025Article
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- The Investigation of Giardiasis (Foodborne and Waterborne Diseases) in Buffaloes in Van Region, Türkiye: First Molecular Report of Giardia duodenalis Assemblage B from BuffaloesPathogens (Basel, Switzerland) · 2023Article
- Zoonotic giardiasis: an update.Parasitology research · 2021Review
- Prevalence and multilocus genotyping of Cryptosporidium spp. in cattle in Jiangxi Province, southeastern China.Parasitology research · 2021Article
- The Age-RelatedAnimals : an open access journal from MDPI · 2021Article
- Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Cryptosporidiosis and Giardiasis in Buffaloes (Frontiers in veterinary science · 2020Review
- First description ofFood and waterborne parasitology · 2019Article
- Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in pre-weaned dairy calves in Guangdong, China.Parasites & vectors · 2019Article
- Molecular characterisation and risk factor analysis of Cryptosporidium spp. in calves from Italy.Parasitology research · 2018Article
- Estimating Cryptosporidium and Giardia disease burdens for children drinking untreated groundwater in a rural population in India.PLoS neglected tropical diseases · 2018Article
- The zoonotic potential of Giardia intestinalis assemblage E in rural settings.Parasitology research · 2016Article
- Prevalence and first genotyping of Giardia duodenalis in beef calves in Vietnam.Tropical animal health and production · 2016Article
- First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia.Parasitology research · 2016Article
- Multilocus genotyping of Giardia duodenalis and Enterocytozoon bieneusi in dairy and native beef (Qinchuan) calves in Shaanxi province, northwestern China.Parasitology research · 2016Article
- Cryptosporidium spp., Giardia intestinalis, and Enterocytozoon bieneusi in Captive Non-Human Primates in Qinling Mountains.The Korean journal of parasitology · 2015Article
- A perspective on Cryptosporidium and Giardia, with an emphasis on bovines and recent epidemiological findings.Advances in parasitology · 2015Review
- Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Ningxia, northwestern China.BMC veterinary research · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe genetic characterization of Cryptosporidium and Giardia has important implications for investigating their epidemiology and underpins their control. We undertook the first molecular epidemiological survey of domestic bovids in selected regions of Sri Lanka to establish whether they excreted Cryptosporidium and/or Giardia with zoonotic potential.
methodsFaecal samples were collected from dairy calves (n = 340; Bos taurus; < 3 months of age; weekly sampling for six weeks) and water buffaloes (n = 297; Bubalus bubalis; <6 months and ≥6 months of age; one sampling) from seven different farms in Sri Lanka. Genomic DNAs were extracted from individual faecal samples and then tested for the presence of parasite DNA using a PCR-based mutation scanning-targeted sequencing-phylogenetic approach, employing genetic markers within the small subunit of nuclear ribosomal RNA and 60 kDa glycoprotein genes (designated pSSU and pgp60, respectively) for Cryptosporidium, and within the triose phosphate isomerise (ptpi) gene for Giardia.
resultsBased on pSSU sequence data, C. bovis, C. ryanae and six new genotypes that were genetically similar but not identical to C. andersoni (n = 1), C. bovis (n = 1), C. ryanae (n = 3) and C. suis (n = 1) were recorded in cattle. For pSSU, two other, new genotypes were defined in water buffalo, which were genetically most similar to Cryptosporidium genotypes recorded previously in this host species in other countries including Australia. Consistent with the findings for pSSU, no species or genotypes of Cryptosporidium with zoonotic potential were detected using pgp60. Based on ptpi sequence data, G. duodenalis assemblages A and E were detected in four and 137 samples from cattle, respectively, and assemblage E in two samples from water buffaloes.
conclusionsThe present study showed that C. parvum, the most commonly reported zoonotic species of Cryptosporidium recognised in bovine calves globally, was not detected in any of the samples from pre-weaned calves tested in the present study. However, eight new genotypes were recorded. Future studies of different host species in various regions are required to investigate the molecular epidemiology of cryptosporidiosis and giardiasis in Sri Lanka and neighbouring countries in South Asia.
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