ArticleGenetics2014
Insight into insulin secretion from transcriptome and genetic analysis of insulin-producing cells of Drosophila.
Article in Genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Molecular and neural basis of vomiting behavior inScience advances · 2025Article
- Tet controls axon guidance in early brain development through glutamatergic signaling.iScience · 2024Article
- Internal sensory neurons regulate stage-specific growth in Drosophila.Development (Cambridge, England) · 2022Article
- Genome-wide analysis identifies Homothorax and Extradenticle as regulators of insulin in Drosophila Insulin-Producing cells.PLoS genetics · 2022Article
- Exploring the link between Parkinson's disease and type 2 diabetes mellitus in Drosophila.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- The Statin Target Hmgcr Regulates Energy Metabolism and Food Intake through Central Mechanisms.Cells · 2022Article
- The Genetics of Diabetes: What We Can Learn fromInternational journal of molecular sciences · 2021Review
- Cell-type-specific, multicolor labeling of endogenous proteins with split fluorescent protein tags inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Glial and Neuronal Neuroglian, Semaphorin-1a and Plexin A Regulate Morphological and Functional Differentiation ofFrontiers in endocrinology · 2021Article
- The antimicrobial peptide defensin cooperates with tumour necrosis factor to drive tumour cell death ineLife · 2019Article
- Intestinal lysozyme liberates Nod1 ligands from microbes to direct insulin trafficking in pancreatic beta cells.Cell research · 2019Article
- Discovering human diabetes-risk gene function with genetics and physiological assays.Nature communications · 2018Article
- dTBC1D7 regulates systemic growth independently of TSC through insulin signaling.The Journal of cell biology · 2018Article
- The sex of specific neurons controls female body growth in Drosophila.PLoS biology · 2017Article
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- Using Drosophila to discover mechanisms underlying type 2 diabetes.Disease models & mechanisms · 2016Review
- Insulin/IGF signaling in Drosophila and other insects: factors that regulate production, release and post-release action of the insulin-like peptides.Cellular and molecular life sciences : CMLS · 2016Review
- Obesity-linked homologues TfAP-2 and Twz establish meal frequency in Drosophila melanogaster.PLoS genetics · 2014Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Insulin-producing cells (IPCs) in the Drosophila brain produce and release insulin-like peptides (ILPs) to the hemolymph. ILPs are crucial for growth and regulation of metabolic activity in flies, functions analogous to those of mammalian insulin and insulin-like growth factors (IGFs). To identify components functioning in IPCs to control ILP production, we employed genomic and candidate gene approaches. We used laser microdissection and messenger RNA sequencing to characterize the transcriptome of larval IPCs. IPCs highly express many genes homologous to genes active in insulin-producing β-cells of the mammalian pancreas. The genes in common encode ILPs and proteins that control insulin metabolism, storage, secretion, β-cell proliferation, and some not previously linked to insulin production or β-cell function. Among these novelties is unc-104, a kinesin 3 family gene, which is more highly expressed in IPCs compared to most other neurons. Knockdown of unc-104 in IPCs impaired ILP secretion and reduced peripheral insulin signaling. Unc-104 appears to transport ILPs along axons. As a complementary approach, we tested dominant-negative Rab genes to find Rab proteins required in IPCs for ILP production or secretion. Rab1 was identified as crucial for ILP trafficking in IPCs. Inhibition of Rab1 in IPCs increased circulating sugar levels, delayed development, and lowered weight and body size. Immunofluorescence labeling of Rab1 showed its tight association with ILP2 in the Golgi of IPCs. Unc-104 and Rab1 join other proteins required for ILP transport in IPCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.