ArticleThe Journal of biological chemistry2014
The perforin pore facilitates the delivery of cationic cargos.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Cytotoxic T Cells: Kill, Memorize, and Mask to Maintain Immune Homeostasis.International journal of molecular sciences · 2025Review
- Single-cell RNA sequencing of baseline PBMCs predicts ICI efficacy and irAE severity in patients with NSCLC.Journal for immunotherapy of cancer · 2025Article
- PriA is involved in Pleurotus ostreatus development and defense against Pseudomonas tolaasii.Antonie van Leeuwenhoek · 2023Article
- Exploiting autophagy balance in T and NK cells as a new strategy to implement adoptive cell therapies.Molecular cancer · 2023Review
- Tackling antimicrobial stewardship through synergy and antimicrobial peptides.RSC medicinal chemistry · 2022Review
- The pore conformation of lymphocyte perforin.Science advances · 2022Article
- Radiotherapy transiently reduces the sensitivity of cancer cells to lymphocyte cytotoxicity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Antimicrobial peptides: Application informed by evolution.Science (New York, N.Y.) · 2020Review
- Synergy and remarkable specificity of antimicrobial peptides in vivo using a systematic knockout approach.eLife · 2019Article
- A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors.Theranostics · 2019Article
- Granzyme K-deficient mice show no evidence of impaired antiviral immunity.Immunology and cell biology · 2017Article
- The mystery behind membrane insertion: a review of the complement membrane attack complex.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Finding a Balance between Protection and Pathology: The Dual Role of Perforin in Human Disease.International journal of molecular sciences · 2017Review
- Perforin Promotes Amyloid Beta Internalisation in Neurons.Molecular neurobiology · 2017Article
- The Membrane Attack Complex/Perforin Superfamily.Journal of molecular microbiology and biotechnology · 2017Article
- Proinflammatory Caspase A Activation and an Antiviral State Are Induced by a Zebrafish Perforin after Possible Cellular and Functional Diversification from a Myeloid Ancestor.Journal of innate immunity · 2016Article
- Analysis of Perforin Assembly by Quartz Crystal Microbalance Reveals a Role for Cholesterol and Calcium-independent Membrane Binding.The Journal of biological chemistry · 2015Article
- Human antimicrobial peptides and proteins.Pharmaceuticals (Basel, Switzerland) · 2014Article
- Perforin competent CD8 T cells are sufficient to cause immune-mediated blood-brain barrier disruption.PloS one · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytotoxic lymphocytes eliminate virally infected or neoplastic cells through the action of cytotoxic proteases (granzymes). The pore-forming protein perforin is essential for delivery of granzymes into the cytoplasm of target cells; however the mechanism of this delivery is incompletely understood. Perforin contains a membrane attack complex/perforin (MACPF) domain and oligomerizes to form an aqueous pore in the plasma membrane; therefore the simplest (and best supported) model suggests that granzymes passively diffuse through the perforin pore into the cytoplasm of the target cell. Here we demonstrate that perforin preferentially delivers cationic molecules while anionic and neutral cargoes are delivered inefficiently. Furthermore, another distantly related pore-forming MACPF protein, pleurotolysin (from the oyster mushroom), also favors the delivery of cationic molecules, and efficiently delivers human granzyme B. We propose that this facilitated diffusion is due to conserved features of oligomerized MACPF proteins, which may include an anionic lumen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.