ArticleThe Journal of biological chemistry2014
A bifunctional glycosyltransferase from Agrobacterium tumefaciens synthesizes monoglucosyl and glucuronosyl diacylglycerol under phosphate deprivation.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- The phospholipase PlcP mediates membrane lipid remodeling required for the intracellular survival of Burkholderia cenocepacia.The Journal of biological chemistry · 2026Article
- Mycobacterium tuberculosis overcomes phosphate starvation by extensively remodelling its lipidome with phosphorus-free lipids.Nature communications · 2025Article
- Biosurfactant biosynthesis by Alcanivorax borkumensis and its role in oil biodegradation.Nature chemical biology · 2025Article
- Critical parameters for robustPlant direct · 2024Article
- The Proteobacterial MethanotrophmBio · 2022Article
- Phosphorus stress induces the synthesis of novel glycolipids in Pseudomonas aeruginosa that confer protection against a last-resort antibiotic.The ISME journal · 2021Article
- A Glycolipid Glycosyltransferase with Broad Substrate Specificity from the Marine Bacterium "Applied and environmental microbiology · 2021Article
- Are Cyanobacteria an Ancestor of Chloroplasts or Just One of the Gene Donors for Plants and Algae?Genes · 2021Article
- Two bacterial glycosphingolipid synthases responsible for the synthesis of glucuronosylceramide and α-galactosylceramide.The Journal of biological chemistry · 2020Article
- Article
- Lipid remodelling is a widespread strategy in marine heterotrophic bacteria upon phosphorus deficiency.The ISME journal · 2016Article
- System-Wide Adaptations of Desulfovibrio alaskensis G20 to Phosphate-Limited Conditions.PloS one · 2016Article
- SAR11 lipid renovation in response to phosphate starvation.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Accumulation of novel glycolipids and ornithine lipids in Mesorhizobium loti under phosphate deprivation.Journal of bacteriology · 2015Article
- LipidBlast templates as flexible tools for creating new in-silico tandem mass spectral libraries.Analytical chemistry · 2014Article
- Membrane lipids in Agrobacterium tumefaciens: biosynthetic pathways and importance for pathogenesis.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycolipids are mainly found in phototrophic organisms (like plants and cyanobacteria), in Gram-positive bacteria, and a few other bacterial phyla. Besides the function as bulk membrane lipids, they often play a role under phosphate deprivation as surrogates for phospholipids. The Gram-negative Agrobacterium tumefaciens accumulates four different glycolipids under phosphate deficiency, including digalactosyl diacylglycerol and glucosylgalactosyl diacylglycerol synthesized by a processive glycosyltransferase. The other two glycolipids have now been identified by mass spectrometry and nuclear magnetic resonance spectroscopy as monoglucosyl diacylglycerol and glucuronosyl diacylglycerol. These two lipids are synthesized by a single promiscuous glycosyltransferase encoded by the ORF atu2297, with UDP-glucose or UDP-glucuronic acid as sugar donors. The transfer of sugars differing in their chemistry is a novel feature not observed before for lipid glycosyltransferases. Furthermore, this enzyme is the first glucuronosyl diacylglycerol synthase isolated. Deletion mutants of Agrobacterium lacking monoglucosyl diacylglycerol and glucuronosyl diacylglycerol or all glycolipids are not impaired in growth or virulence during infection of tobacco leaf discs. Our data suggest that the four glycolipids and the nonphospholipid diacylglyceryl trimethylhomoserine can mutually replace each other during phosphate deprivation. This redundancy of different nonphospholipids may represent an adaptation mechanism to enhance the competitiveness in nature.
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